Detailed information of TA system TA179251

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 69971..70698
• Replicon: plasmid pBS433-2
• Accession: NZ_CP063891
• Organism: Klebsiella pneumoniae strain BS433-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: ISB10_RS26630
• Protein ID: WP_011251285.1
• Coordinates: 70387..70698 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ISB10_RS26625
• Protein ID: WP_011251286.1
• Coordinates: 69971..70390 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISB10_RS26595	65139..65609	-	471	WP_048333570.1	hypothetical protein	-
ISB10_RS26600	65802..66557	-	756	Protein_66	hypothetical protein	-
ISB10_RS26605	67153..67596	+	444	WP_048333569.1	hypothetical protein	-
ISB10_RS26610	67617..68405	+	789	WP_040217257.1	hypothetical protein	-
ISB10_RS26615	68419..68754	+	336	Protein_69	conjugal transfer protein TraI	-
ISB10_RS26620	68856..69824	-	969	WP_074428168.1	IS5 family transposase	-
ISB10_RS26625	69971..70390	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
ISB10_RS26630	70387..70698	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
ISB10_RS26635	70903..71361	-	459	Protein_73	DDE-type integrase/transposase/recombinase	-
ISB10_RS26640	71475..72172	+	698	Protein_74	IS1 family transposase	-
ISB10_RS26645	73558..74507	-	950	Protein_75	DUF1173 family protein	-
ISB10_RS26650	74685..75179	+	495	WP_004212794.1	thermonuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA	1..227749	227749
	-	inside	IScluster/Tn	-	-	59851..72172	12321

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T179251 WP_011251285.1 NZ_CP063891:c70698-70387 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T179251 NZ_CP084654:c4512365-4512045 [Pectobacterium versatile]
ATGCAACCATCACCTACAATCCCGTCACGGGAGGATAACCCCTGTGCGTCACCTATAGCTGTCTGGCAGCAGCTTCTGAC
CTACCTGCTGGAAAAGCACTACGGCCTCACGCTGAACTATACGCCGTTCTGTGAGGAAAATGTGATTCAGGAGCATATCG
ATGCTGGCATCACGTTGGTCAATGCCGTGAATTTTCTGGTGGAAAAATACGAACTTGTGCGTATCGATCGCAACGGTTTT
AACTGGCAGGAGCAATCGCCATTTCTCACTGCTGTTGATGTTCTCCGGGCCAGACGTGCTACCGGCTTGCTCAAAGCATA
A

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT179251 WP_011251286.1 NZ_CP063891:c70390-69971 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT179251 NZ_CP084654:c4512770-4512435 [Pectobacterium versatile]
ATGCCATCATCGGATACCCCTGAATGGGGACTAAAATGTGCCATCACTCCGCGATTCGGAGCTAGGCTGGTGCAGGAAGG
TAACCGTCTGCATTATCTGGCAGATCGGGCCAGCGTTGTCGGTACGTTCAGCAAAACAGGAGCCCAAAGTCTGGAACGTT
GCTTCCCTGAGCTGATTAAGCAGTTGGAGCAGAAGCTACGCACGGGTGAACTGAATCCCCGCCAGCAGGGTTGTGTCACG
TTGCATTGCAACGAATGGGCCTGTGAAGCTGATACGCTGGGCAGTGTTGGTTACGTGTATATCGTTATTTATCCAACTTC
TGCTGCAACACTGTAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1