Detailed information of TA system TA179179

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 4883010..4883699
• Replicon: chromosome
• Accession: NZ_CP063881
• Organism: Klebsiella pneumoniae strain S90-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A331C6E2
• Locus tag: IOD49_RS23950
• Protein ID: WP_021469727.1
• Coordinates: 4883010..4883327 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IOD49_RS23955
• Protein ID: WP_040147778.1
• Coordinates: 4883403..4883699 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOD49_RS23920	4878712..4879221	+	510	WP_002906702.1	GNAT family N-acetyltransferase	-
IOD49_RS23925	4879231..4880157	+	927	WP_102000933.1	amino acid ABC transporter permease	-
IOD49_RS23930	4880141..4880920	+	780	WP_102000934.1	amino acid ABC transporter ATP-binding protein	-
IOD49_RS23935	4880959..4881810	+	852	WP_014343117.1	transporter substrate-binding domain-containing protein	-
IOD49_RS23940	4881888..4882505	+	618	WP_004175955.1	glutathione S-transferase family protein	-
IOD49_RS23945	4882576..4882803	+	228	WP_002906690.1	tautomerase PptA	-
IOD49_RS23950	4883010..4883327	+	318	WP_021469727.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IOD49_RS23955	4883403..4883699	+	297	WP_040147778.1	helix-turn-helix domain-containing protein	Antitoxin
IOD49_RS23960	4883778..4884224	+	447	WP_004175959.1	hypothetical protein	-
IOD49_RS23965	4884265..4885881	-	1617	WP_040147777.1	carbohydrate porin	-
IOD49_RS23970	4885925..4887307	-	1383	WP_004151222.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-76)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12221.10 Da
• Isoelectric Point: 10.2217

>T179179 WP_021469727.1 NZ_CP063881:4883010-4883327 [Klebsiella pneumoniae]
MLTFIELQGFSKRRQVLLQDDEFRAFQEILINDPEAGDTISGTGGFRKIRWSRPGMGKRGGVRVIYYNVTKKGRIYLALI
YPKNEQDELNQEQKKMLRQVADLIN

Nucleotide

• Length: 318 bp

>T179179 NZ_CP084641:2783379-2783582 [Yersinia ruckeri]
ATGACCAAAACTGACTACCTAATGCGTTTAAGAAAATGCACAACAATTGACACACTAGAGCGTGTTATTGAAAAAAATAA
GTATGAGCTATCTGATGATGAACTAGAATTATTCTATTCTGCTGCGGATCATCGTCTAGCTGAACTTACCATGAATAAGT
TATACGATAAAATTCCTGCAACTGTCTGGCAACATGTGAAGTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11032.88 Da
• Isoelectric Point: 6.4721

>AT179179 WP_040147778.1 NZ_CP063881:4883403-4883699 [Klebsiella pneumoniae]
MKDELFADLLASAEEMVRIEKGEQMPMEQHVHTFSEIDVKAIREATGLKQQDFAAAVGVSYELVKSWETKRRQPTGAARK
LLLLLQGNPLIINLLKVV

Nucleotide

• Length: 297 bp

>AT179179 NZ_CP084641:2782959-2783327 [Yersinia ruckeri]
ATGGATGAGTACTCACCTAAGCGACATGATATTGCTCAGTTGAAATTCTTATGTGAGAATTTGTATGATGAAGGTATCGC
CACATTGGGCGATAGCCACCACGGCTGGGTTAATGATCCAACCTCCGCGGTAAATCTCCAACTGAATGATTTAATCGAAC
ACATAGCTTCATTTGTGATGAGTTTTAAAATAAAATACGCAGACGAAATCGATCTCTGCGAGTTGGTGGAGGAATATCTA
GACGATACATATACCTTGTTCAGCAGTTACGGAATCAATGATCCTGATTTACGCCGCTGGCAGAAAACAAAAGACCGATT
ATTCAGACTATTTTCAGGGGAATACCTATGTGCCCTGATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A331C6E2