Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 58135..58862 Replicon plasmid pM169-3.1
Accession NZ_CP063879
Organism Klebsiella pneumoniae strain M169-3

Toxin (Protein)


Gene name higB Uniprot ID A0A5Q9LNL2
Locus tag ISB47_RS27370 Protein ID WP_004118600.1
Coordinates 58551..58862 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag ISB47_RS27365 Protein ID WP_000990392.1
Coordinates 58135..58554 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISB47_RS27330 53822..54184 - 363 WP_020324593.1 arsenite efflux transporter metallochaperone ArsD -
ISB47_RS27335 54232..54585 - 354 WP_001114073.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
ISB47_RS27340 54878..55018 + 141 WP_004118614.1 hypothetical protein -
ISB47_RS27345 55186..55494 + 309 WP_020324596.1 Ref family recombination enhancement nuclease -
ISB47_RS27350 55918..56577 + 660 WP_000078540.1 hypothetical protein -
ISB47_RS27355 56574..56903 + 330 WP_004118613.1 hypothetical protein -
ISB47_RS27360 56896..58098 + 1203 WP_003031541.1 hypothetical protein -
ISB47_RS27365 58135..58554 - 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
ISB47_RS27370 58551..58862 - 312 WP_004118600.1 type II toxin-antitoxin system HigB family toxin Toxin
ISB47_RS27375 59073..59453 + 381 Protein_67 gluconate permease -
ISB47_RS27380 59503..60198 - 696 WP_004118595.1 lactate utilization protein C -
ISB47_RS27385 60191..61618 - 1428 WP_000023895.1 LutB/LldF family L-lactate oxidation iron-sulfur protein -
ISB47_RS27390 61629..62348 - 720 WP_001102107.1 (Fe-S)-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 - 1..131081 131081


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12386.14 Da        Isoelectric Point: 10.0935

>T179157 WP_004118600.1 NZ_CP063879:c58862-58551 [Klebsiella pneumoniae]
MHIVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T179157 NZ_CP084625:1276757-1277326 [Pseudomonas lalkuanensis]
ATGTTCAATCCACAACCGGTCACCCTGCAGCGCGGCGCATTGCGCCTCGAGCCGCTGATGGAGGCCGATATCCCGGCGCT
GGTGACCCTGGCCGAAGCCAACCGCGAAGTCCTGGTCTACATGAATGGTCCGCTGTGGCCCGACTGGTATCGCCAGGCCC
TGGCCGCCCAGCGGGAAGGTCGGGCAGTGGTCTTCGCCATCCGCCTGGGCGACAACCTGGTGGGTACCACGCGCTTCGCC
GATTTCATGCCCAGCCTGCCGGCCGCCGAAATCGGCTGGACCTGGCTGGCCGCCGGCGAGCATGGCAGCGGCCTGAACGC
CTCGATCAAGTACCTGATGCTGCGCCACGCCTTCGAAGAGTGGCAGCTGGTGCGGGTGCAACTGAAGACCGCCGCCAGCA
ACCTGCGCTCCCAGCGAGCCATCGAGAAACTGGGCGCGCAGCGCGAGGGTGTGCTGCGCAACCATCGCCGCCTGGCCGAC
GGCCGCCTCGACGACAGCGTGCTCTACAGCATCACCGACCACGACTGGCCGGAGGTCAGGCAACGCCTGGAGGCGCTGTT
CAGCGCATAG

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT179157 WP_000990392.1 NZ_CP063879:c58554-58135 [Klebsiella pneumoniae]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT179157 NZ_CP084625:1277329-1278171 [Pseudomonas lalkuanensis]
GTGAGCGAGCAGAGGGAAGACGCGCATTTCTGGCAGGCGGGCCATCTGGATGGCCTGGAGCTGTTGTCTGCACGCTATAT
AGAACATCGCTTCGCGCCCCATGTGCATGACGGCTATGTGATCGCCGTGGTGGAGGCGGGTGCCGAGCGCTACCGCTATC
GCGGCGTCGATCACCTGGCCGGTGCCGGCAGCCTGGCGTTGATCAACCCGGATGAGGTGCACACCGGCTCCAAGGGGCAC
GACGACGGCTGGCGCTACCGGGTGTTCTATCCGGATGTCGCCCTGGTGCGCAGTGCCCTTGAAGAACTCGAACTGCCGCT
GGCCGGGATGCCGGCCTTCATCGGTAGCGTTCACCACGATCCCGACCTGGTCCGGATGTTCACTCATCTCCATCGGTTGC
TGGAAGCGAATGCCAGTGCCCTGGAGCAACAGACAGCCTGGCGCGAGGCCCTGCTGGCGCTGTTCCGTCGCCATTCCAGA
TTGCCTGAACCCGGCGCTCCTGGTCGCGAGCCCCTGGCCGTGTTGCACGCCAAGGAACTGCTGCGCGAACGCCTGGCCGA
GCCGCCGTCCCTTGAGGAGCTGGCCAGCGCGGTGAGGCTCTCGCCCTTCCACTTCGCCCGCACGTTCCGCCGGTCCACCG
GCATGCCGCCGCACGCCTGGCTCAAGCAACAGCGTCTGGACCAGGCCCGGGCCCTGCTCAGGGCCGGCTGCACACCAGCC
GGCGTGGCAGCCCAGCTGGGCTTCGCCGACCAGAGCCACCTGACCCGTCAGTTCAAGCAGGCCTATGGCGTGGGACCGGG
CGAATATCGCAACGCCTGCGCAAGATCGTTCAAGACCCATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LNL2

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References