Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 5396976..5397573 Replicon chromosome
Accession NZ_CP063878
Organism Klebsiella pneumoniae strain M169-3

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag ISB47_RS26625 Protein ID WP_004142563.1
Coordinates 5397256..5397573 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag ISB47_RS26620 Protein ID WP_004142561.1
Coordinates 5396976..5397263 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISB47_RS26590 (5393057) 5393057..5393305 + 249 WP_002893037.1 DUF1158 domain-containing protein -
ISB47_RS26595 (5393323) 5393323..5393664 - 342 WP_064181824.1 RamA family antibiotic efflux transcriptional regulator -
ISB47_RS26600 (5393695) 5393695..5394810 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
ISB47_RS26605 (5394989) 5394989..5395570 + 582 WP_032419262.1 TetR/AcrR family transcriptional regulator -
ISB47_RS26610 (5395570) 5395570..5395938 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
ISB47_RS26615 (5396058) 5396058..5396711 + 654 WP_032419263.1 oxygen-insensitive NAD(P)H nitroreductase -
ISB47_RS26620 (5396976) 5396976..5397263 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
ISB47_RS26625 (5397256) 5397256..5397573 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ISB47_RS26630 (5397758) 5397758..5398801 - 1044 WP_032419264.1 DUF2157 domain-containing protein -
ISB47_RS26635 (5399468) 5399468..5400334 - 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
ISB47_RS26640 (5400443) 5400443..5401870 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T179156 WP_004142563.1 NZ_CP063878:c5397573-5397256 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T179156 NZ_CP084622:228293-228643 [Mesobacillus sp. S13]
TTGATTGTCAAACGTGGTGACGTCTATTTTGCGGACCTATCCCCAGTTGTTGGTTCAGAACAAGGCGGAGTTCGCCCTGT
CCTGGTCATCCAAAACGACATCGGGAATCGGTTTAGTCCCACAGTCATTATCGCAGCGATCACAGCACAGATCCAAAAAG
CCAAGCTTCCCACTCATGTAGAAATAGATGCGAAGCGGTACGGTTTTGAACGAGACTCGGTCATCTTATTGGAGCAAATA
CGCACAATCGACAAACAGCGCCTAACCGATAAAATTACCCATCTCGATGACGAAATGATGGAAAAAGTGGATGAAGCCCT
TCAGGTAAGTTTAGGTCTTATCGAATTTTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT179156 WP_004142561.1 NZ_CP063878:c5397263-5396976 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT179156 NZ_CP084622:228007-228288 [Mesobacillus sp. S13]
GTGTCTGAATCCAGCGCAACAACTGAGATTTTGGTAAAGTTACCGCAGCATCTTTTAAGTGAACTAGATGGATTTGTTAA
GCAAGAGAACGTAAACCGCAGCGAATTTATTTATCAGGCAACAAAAATGTTTTTGCGTGAGAAGAAAAAGAGACAAATTC
GTGAATCCATGAGACGTGGCTATATGGAAATGGCCAAGATTAATCTGACCATTGCATCTGAAGCATTTCAAGCAGAATAC
GAGGCAGAACACACAGTTGAACGTCTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References