Detailed information of TA system TA179088

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4287593..4288239
• Replicon: chromosome
• Accession: NZ_CP063867
• Organism: Klebsiella variicola strain M142-3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: ISD32_RS20465
• Protein ID: WP_012540399.1
• Coordinates: 4287593..4287940 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1F2LZT5
• Locus tag: ISD32_RS20470
• Protein ID: WP_008806992.1
• Coordinates: 4287940..4288239 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISD32_RS20455	4283480..4284913	+	1434	WP_004202133.1	glycogen synthase GlgA	-
ISD32_RS20460	4284931..4287378	+	2448	WP_008806990.1	glycogen phosphorylase	-
ISD32_RS20465	4287593..4287940	+	348	WP_012540399.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ISD32_RS20470	4287940..4288239	+	300	WP_008806992.1	helix-turn-helix transcriptional regulator	Antitoxin
ISD32_RS20475	4288302..4289810	-	1509	WP_110224113.1	glycerol-3-phosphate dehydrogenase	-
ISD32_RS20480	4290015..4290344	+	330	WP_004202138.1	thiosulfate sulfurtransferase GlpE	-
ISD32_RS20485	4290395..4291225	+	831	WP_008806994.1	rhomboid family intramembrane serine protease GlpG	-
ISD32_RS20490	4291275..4292033	+	759	WP_008806995.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13505.50 Da
• Isoelectric Point: 5.6749

>T179088 WP_012540399.1 NZ_CP063867:4287593-4287940 [Klebsiella variicola]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFGPQLGRPYVDTVKDSTFQDMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T179088 NZ_CP084532:200452-200937 [Salmonella enterica subsp. enterica serovar Enteritidis]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGTGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10814.61 Da
• Isoelectric Point: 10.1464

>AT179088 WP_008806992.1 NZ_CP063867:4287940-4288239 [Klebsiella variicola]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT179088 NZ_CP084532:200177-200464 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCAGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1F2LZT5