Detailed information of TA system TA179068

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 4611264..4611861
• Replicon: chromosome
• Accession: NZ_CP063865
• Organism: Klebsiella pneumoniae strain S130-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: IOD48_RS22645
• Protein ID: WP_004893639.1
• Coordinates: 4611264..4611581 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: IOD48_RS22650
• Protein ID: WP_004142561.1
• Coordinates: 4611574..4611861 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOD48_RS22630	4606965..4608391	-	1427	Protein_4419	MFS transporter	-
IOD48_RS22635	4608500..4609366	+	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
IOD48_RS22640	4610036..4611079	+	1044	WP_023279204.1	DUF2157 domain-containing protein	-
IOD48_RS22645	4611264..4611581	+	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IOD48_RS22650	4611574..4611861	+	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
IOD48_RS22655	4611999..4612652	-	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
IOD48_RS22660	4612772..4613140	-	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
IOD48_RS22665	4613140..4613721	-	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
IOD48_RS22670	4613901..4615016	+	1116	WP_074180844.1	MBL fold metallo-hydrolase	-
IOD48_RS22675	4615047..4615388	+	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
IOD48_RS22680	4615406..4615654	-	249	WP_002893037.1	DUF1158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T179068 WP_004893639.1 NZ_CP063865:4611264-4611581 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T179068 NZ_CP084529:2224683-2224940 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT179068 WP_004142561.1 NZ_CP063865:4611574-4611861 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT179068 NZ_CP084529:2224952-2225497 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1