Detailed information of TA system TA179037

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1905578..1906224
• Replicon: chromosome
• Accession: NZ_CP063863
• Organism: Klebsiella pneumoniae strain M63-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: IOD47_RS08890
• Protein ID: WP_032415766.1
• Coordinates: 1905578..1905925 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: W8UB68
• Locus tag: IOD47_RS08895
• Protein ID: WP_002920557.1
• Coordinates: 1905925..1906224 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOD47_RS08880	1901504..1902937	+	1434	WP_002920564.1	glycogen synthase GlgA	-
IOD47_RS08885	1902955..1905402	+	2448	WP_200995594.1	glycogen phosphorylase	-
IOD47_RS08890	1905578..1905925	+	348	WP_032415766.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IOD47_RS08895	1905925..1906224	+	300	WP_002920557.1	helix-turn-helix transcriptional regulator	Antitoxin
IOD47_RS08900	1906287..1907795	-	1509	WP_002920554.1	glycerol-3-phosphate dehydrogenase	-
IOD47_RS08905	1908000..1908329	+	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
IOD47_RS08910	1908380..1909210	+	831	WP_004151408.1	rhomboid family intramembrane serine protease GlpG	-
IOD47_RS08915	1909260..1910018	+	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (9-87)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13535.49 Da
• Isoelectric Point: 5.2054

>T179037 WP_032415766.1 NZ_CP063863:1905578-1905925 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNENRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T179037 NZ_CP084504:c81304-80981 [Klebsiella pneumoniae]
ATGAACTATCTGGAGTTTGTTGAAACCAGCGCTTTCAGTGGACTGCGTAAGGAACTCATGGATGATGATGAATTCCGCGA
ACTGCAGACCTATTTACTGGAAGCCCATGATCGGGGTGACACCATCAGTCATACCGGTGGATGCAGGAAGATTCGCTGGG
GTCGCCCGGGGATGGGTAAGCGAGGTGGCGTCAGGGTGATTTACTACGTTCGTCTTGCCAGCGGCAGACTGTATCTGCTG
CTGATATACCCGAAGAATGCGAAAGATGAGCTCAGCGAGAAAGAAAAAGCCGTTATGAAGGCATTAACACAGCAGCTGAA
GTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10800.58 Da
• Isoelectric Point: 10.1464

>AT179037 WP_002920557.1 NZ_CP063863:1905925-1906224 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT179037 NZ_CP084504:c80932-80630 [Klebsiella pneumoniae]
ATGGAAAAGAAACTTTTTGATGCCCTGATCAGCAGCATGGAGGAAATGGTGGCCATTGAAAATGGCCAGATCCAGCCCGC
TGCAGAACAGGTTCACCGCCATACACTGCCGGATGTGAAAGCCATTCGTAAACATGCCGGCATGAAACAGGCTGAATTTG
CAGAAGTGGTGGGGGCCAGCGTTGACCTTGTTCGCAGCTGGGAACAGCAACGGCGTGTTCCGTCGGGTATTGCGCTGAAA
ATGCTCTGCCTCATCGAGCGTGAGCCTGCACTGATTAATACGTTGCGTGGTATTCACGTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E1CBF8