Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 4165886..4166472 Replicon chromosome
Accession NZ_CP063856
Organism Klebsiella pneumoniae strain N1059-5At

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag IOD44_RS19905 Protein ID WP_002920800.1
Coordinates 4166104..4166472 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag IOD44_RS19900 Protein ID WP_004174006.1
Coordinates 4165886..4166107 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IOD44_RS19880 4162043..4162969 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
IOD44_RS19885 4162966..4164243 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
IOD44_RS19890 4164240..4165007 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
IOD44_RS19895 4165009..4165722 + 714 WP_101987001.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
IOD44_RS19900 4165886..4166107 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
IOD44_RS19905 4166104..4166472 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
IOD44_RS19910 4166745..4168061 + 1317 WP_094488153.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
IOD44_RS19915 4168168..4169055 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
IOD44_RS19920 4169052..4169897 + 846 WP_004185988.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
IOD44_RS19925 4169899..4170969 + 1071 WP_094488154.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4162966..4171706 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T179001 WP_002920800.1 NZ_CP063856:4166104-4166472 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T179001 NZ_CP084493:c111424-110981 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT179001 WP_004174006.1 NZ_CP063856:4165886-4166107 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT179001 NZ_CP084493:c111651-111421 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References