Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 2425942..2426539 Replicon chromosome
Accession NZ_CP063856
Organism Klebsiella pneumoniae strain N1059-5At

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag IOD44_RS11790 Protein ID WP_004142563.1
Coordinates 2426222..2426539 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag IOD44_RS11785 Protein ID WP_004142561.1
Coordinates 2425942..2426229 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IOD44_RS11755 2422022..2422270 + 249 WP_002893037.1 DUF1158 domain-containing protein -
IOD44_RS11760 2422288..2422629 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
IOD44_RS11765 2422660..2423775 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
IOD44_RS11770 2423955..2424536 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
IOD44_RS11775 2424536..2424904 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
IOD44_RS11780 2425024..2425677 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
IOD44_RS11785 2425942..2426229 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
IOD44_RS11790 2426222..2426539 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IOD44_RS11795 2426724..2427767 - 1044 WP_023284821.1 DUF2157 domain-containing protein -
IOD44_RS11800 2428434..2429300 - 867 WP_032105285.1 helix-turn-helix transcriptional regulator -
IOD44_RS11805 2429409..2430836 + 1428 WP_032411778.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T178995 WP_004142563.1 NZ_CP063856:c2426539-2426222 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T178995 NZ_CP084492:c4714603-4714070 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT178995 WP_004142561.1 NZ_CP063856:c2426229-2425942 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT178995 NZ_CP084492:c4714880-4714614 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References