Detailed information of TA system TA178982

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 62979..63405
• Replicon: plasmid pM1023-4Ar.4
• Accession: NZ_CP063855
• Organism: Klebsiella pneumoniae strain M1023-4Ar

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: IOD43_RS27730
• Protein ID: WP_001312861.1
• Coordinates: 63247..63405 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	62979..63203 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOD43_RS27705	58901..59428	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
IOD43_RS27710	59486..59719	+	234	WP_000006003.1	DUF905 family protein	-
IOD43_RS27715	59780..61748	+	1969	Protein_69	ParB/RepB/Spo0J family partition protein	-
IOD43_RS27720	61817..62251	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
IOD43_RS27725	62248..62967	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	62979..63203	+	225	NuclAT_0	-	Antitoxin
-	62979..63203	+	225	NuclAT_0	-	Antitoxin
-	62979..63203	+	225	NuclAT_0	-	Antitoxin
-	62979..63203	+	225	NuclAT_0	-	Antitoxin
IOD43_RS27730	63247..63405	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
IOD43_RS27735	63643..64020	-	378	Protein_73	hypothetical protein	-
IOD43_RS27740	64320..64616	+	297	WP_001272251.1	hypothetical protein	-
IOD43_RS27745	64727..65548	+	822	WP_001234445.1	DUF945 domain-containing protein	-
IOD43_RS27750	65845..66492	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
IOD43_RS27755	66769..67152	+	384	WP_000124981.1	relaxosome protein TraM	-
IOD43_RS27760	67343..68029	+	687	WP_015059009.1	PAS domain-containing protein	-
IOD43_RS27765	68123..68350	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / fosA3 / blaTEM-1B / blaCTX-M-55	-	1..89958	89958

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T178982 WP_001312861.1 NZ_CP063855:63247-63405 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T178982 NZ_CP084484:c4753226-4752948 [Pseudomonas sp. PS1(2021)]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTTGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT178982 NZ_CP063855:62979-63203 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin