Detailed information of TA system TA178979

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 12605..12858
• Replicon: plasmid pM1023-4Ar.4
• Accession: NZ_CP063855
• Organism: Klebsiella pneumoniae strain M1023-4Ar

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: IOD43_RS27415
• Protein ID: WP_001312851.1
• Coordinates: 12709..12858 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	12605..12664 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOD43_RS27395	9332..10078	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
IOD43_RS27400	10133..10693	+	561	WP_000139328.1	fertility inhibition protein FinO	-
IOD43_RS27405	10825..11025	+	201	WP_015059022.1	hypothetical protein	-
IOD43_RS27410	11411..12010	+	600	WP_032083981.1	hypothetical protein	-
-	12605..12664	-	60	NuclAT_1	-	Antitoxin
-	12605..12664	-	60	NuclAT_1	-	Antitoxin
-	12605..12664	-	60	NuclAT_1	-	Antitoxin
-	12605..12664	-	60	NuclAT_1	-	Antitoxin
IOD43_RS27415	12709..12858	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
IOD43_RS27420	13142..13390	+	249	WP_000083837.1	replication regulatory protein RepA	-
IOD43_RS27425	13635..13709	+	75	WP_001375168.1	RepA leader peptide Tap	-
IOD43_RS27430	13702..14559	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
IOD43_RS27435	15498..16151	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
IOD43_RS27440	16244..16501	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
IOD43_RS27445	16434..16835	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
IOD43_RS27450	17084..17499	+	416	Protein_16	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / fosA3 / blaTEM-1B / blaCTX-M-55	-	1..89958	89958

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T178979 WP_001312851.1 NZ_CP063855:12709-12858 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T178979 NZ_CP084484:c3541479-3540724 [Pseudomonas sp. PS1(2021)]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATATCCGACCGCTCCAGGGGCGCCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGCTGGGGG
TCGCGGTTTGGCCGTCGCCACGAGCCAAGCCTGTTCTACGCCGCGTTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGCGTCCTGTGGAGTGGCATGGCGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGAACTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT178979 NZ_CP063855:c12664-12605 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin