Detailed information of TA system TA178933

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 127579..127832
• Replicon: plasmid pCRKP66R-3
• Accession: NZ_CP063835
• Organism: Klebsiella pneumoniae strain CRKP66R

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: IRT47_RS28570
• Protein ID: WP_001312851.1
• Coordinates: 127683..127832 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	127579..127638 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IRT47_RS28530	122961..123305	-	345	Protein_167	IS6-like element IS26 family transposase	-
IRT47_RS28535	123357..124061	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
IRT47_RS28540	124125..124286	+	162	Protein_169	DNA helicase	-
IRT47_RS28545	124306..125052	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
IRT47_RS28550	125107..125667	+	561	WP_000139328.1	fertility inhibition protein FinO	-
IRT47_RS28555	125799..125999	+	201	WP_015059022.1	hypothetical protein	-
IRT47_RS28560	126385..126984	+	600	WP_032083981.1	hypothetical protein	-
IRT47_RS28565	127148..127378	+	231	WP_001736714.1	hypothetical protein	-
-	127579..127638	-	60	NuclAT_1	-	Antitoxin
-	127579..127638	-	60	NuclAT_1	-	Antitoxin
-	127579..127638	-	60	NuclAT_1	-	Antitoxin
-	127579..127638	-	60	NuclAT_1	-	Antitoxin
IRT47_RS28570	127683..127832	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
IRT47_RS28575	128116..128364	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2	-	1..128675	128675

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T178933 WP_001312851.1 NZ_CP063835:127683-127832 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T178933 NZ_CP084429:2934308-2934637 [Edwardsiella piscicida]
ATGCAAACCTTATCATCACACCCGACACGGTCGACTCAGCCCTATCTGTCACCCGTTGAAACCTGGCAACGGCTGCTGAC
TCATCTATTCTCGCAGCACTATGGCCTGACGCTGAATGACACACCGTTCAGTAATGAAACCACCATACGAGAACATATTG
ATGCTGGAGTATCGCTCAGCGATGCGGTGAACTTTCTTGTCGAAAAATACGAGCTTATCCGTATCGATCGGAAGGGATTT
TCGTGGCAACAACAGACTCCGTATATTTCCGTAGTGGATATTCTACGAGCAAGACGCAATACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT178933 NZ_CP063835:c127638-127579 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin