Detailed information of TA system TA178928

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 36981..37407
• Replicon: plasmid pCRKP66R-3
• Accession: NZ_CP063835
• Organism: Klebsiella pneumoniae strain CRKP66R

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: IRT47_RS27935
• Protein ID: WP_001312861.1
• Coordinates: 37249..37407 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	36981..37205 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IRT47_RS27910	32848..33375	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
IRT47_RS27915	33433..33666	+	234	WP_000006003.1	DUF905 family protein	-
IRT47_RS27920	33727..35750	+	2024	Protein_45	ParB/RepB/Spo0J family partition protein	-
IRT47_RS27925	35819..36253	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
IRT47_RS27930	36250..36969	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	36981..37205	+	225	NuclAT_0	-	Antitoxin
-	36981..37205	+	225	NuclAT_0	-	Antitoxin
-	36981..37205	+	225	NuclAT_0	-	Antitoxin
-	36981..37205	+	225	NuclAT_0	-	Antitoxin
IRT47_RS27935	37249..37407	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
IRT47_RS27940	37645..38022	-	378	Protein_49	hypothetical protein	-
IRT47_RS27945	38322..38618	+	297	WP_001272251.1	hypothetical protein	-
IRT47_RS27950	38729..39550	+	822	WP_001234445.1	DUF945 domain-containing protein	-
IRT47_RS27955	39847..40494	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
IRT47_RS27960	40771..41154	+	384	WP_000124981.1	relaxosome protein TraM	-
IRT47_RS27965	41345..42031	+	687	WP_015059009.1	PAS domain-containing protein	-
IRT47_RS27970	42125..42352	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2	-	1..128675	128675

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T178928 WP_001312861.1 NZ_CP063835:37249-37407 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T178928 NZ_CP084428:c1097827-1097624 [Edwardsiella piscicida]
ATGACCAAGATCGATTATCTGATGAAGCTGCGTAAATGCACGACGCTGGATACTCTAGAACGCGTGATCGAAAAGAATAA
ATACGAACTTTCAGATGATGAATTAGAAATTTTTTATTCTGCCGCAGATCATCGTCTGGCCGAATTAACTATGAATAAGC
TATACGACAAAATTCCAGCAGAAGTCTGGCAATACGTCCGCTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT178928 NZ_CP063835:36981-37205 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin