Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2241204..2242123 Replicon chromosome
Accession NZ_CP063804
Organism Mycobacterium orygis strain MUHC/MB/EPTB/Orygis/51145

Toxin (Protein)


Gene name HigB2 Uniprot ID -
Locus tag RJtmp_RS10590 Protein ID WP_003410128.1
Coordinates 2241518..2242123 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag RJtmp_RS10585 Protein ID WP_003410124.1
Coordinates 2241204..2241509 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RJtmp_RS10555 (RJtmp_002084) 2236215..2237471 - 1257 WP_003410095.1 HNH endonuclease signature motif containing protein -
RJtmp_RS10560 (RJtmp_002085) 2237825..2238400 + 576 WP_003410100.1 hypothetical protein -
RJtmp_RS10565 (RJtmp_002086) 2238397..2239437 + 1041 WP_249739967.1 ImmA/IrrE family metallo-endopeptidase -
RJtmp_RS10570 (RJtmp_002087) 2239679..2240398 + 720 WP_003410108.1 DUF433 domain-containing protein -
RJtmp_RS10575 (RJtmp_002088) 2240388..2240804 + 417 WP_003410114.1 hypothetical protein -
RJtmp_RS10580 (RJtmp_002089) 2240820..2241119 - 300 WP_003410120.1 hypothetical protein -
RJtmp_RS10585 (RJtmp_002090) 2241204..2241509 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
RJtmp_RS10590 (RJtmp_002091) 2241518..2242123 - 606 WP_003410128.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
RJtmp_RS10595 (RJtmp_002092) 2242148..2242507 - 360 WP_003410131.1 hypothetical protein -
RJtmp_RS10600 (RJtmp_002093) 2242667..2243257 - 591 WP_249739968.1 hypothetical protein -
RJtmp_RS10605 (RJtmp_002094) 2243296..2243532 - 237 WP_003410133.1 hypothetical protein -
RJtmp_RS10610 (RJtmp_002095) 2243739..2244635 - 897 WP_003410136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22761.00 Da        Isoelectric Point: 7.3073

>T178876 WP_003410128.1 NZ_CP063804:c2242123-2241518 [Mycobacterium orygis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDLARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T178876 NZ_CP084397:2020216-2020318 [Klebsiella pneumoniae subsp. pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT178876 WP_003410124.1 NZ_CP063804:c2241509-2241204 [Mycobacterium orygis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT178876 NZ_CP084397:c2020325-2020180 [Klebsiella pneumoniae subsp. pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References