Detailed information of TA system TA178831

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 2009451..2010055
• Replicon: chromosome
• Accession: NZ_CP063780
• Organism: Pseudomonas sp. OST1909

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: IH404_RS08790
• Protein ID: WP_194440293.1
• Coordinates: 2009741..2010055 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IH404_RS08785
• Protein ID: WP_003189536.1
• Coordinates: 2009451..2009738 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IH404_RS08745	2005211..2005924	+	714	WP_005786030.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
IH404_RS08755	2006219..2006614	+	396	WP_005786031.1	nucleotidyltransferase domain-containing protein	-
IH404_RS08760	2006604..2007023	+	420	WP_194440292.1	DUF86 domain-containing protein	-
IH404_RS08765	2007056..2007136	-	81	Protein_1726	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
IH404_RS08770	2007137..2007247	-	111	Protein_1727	multidrug transporter	-
IH404_RS08775	2007659..2007910	+	252	WP_005786036.1	hypothetical protein	-
IH404_RS08780	2007986..2009212	-	1227	WP_005786037.1	MFS transporter	-
IH404_RS08785	2009451..2009738	-	288	WP_003189536.1	helix-turn-helix domain-containing protein	Antitoxin
IH404_RS08790	2009741..2010055	-	315	WP_194440293.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IH404_RS08795	2010161..2011102	-	942	WP_005786043.1	LysR family transcriptional regulator	-
IH404_RS08800	2011260..2012576	+	1317	WP_005786044.1	MHS family MFS transporter	-
IH404_RS08805	2012588..2013820	+	1233	WP_005786047.1	Zn-dependent hydrolase	-
IH404_RS08810	2013955..2014992	+	1038	WP_005786049.1	histone deacetylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2006604..2020453	13849

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (16-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-86)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11640.48 Da
• Isoelectric Point: 10.0575

>T178831 WP_194440293.1 NZ_CP063780:c2010055-2009741 [Pseudomonas sp. OST1909]
MIFIETSVFTRRVKELIDEDAYTALQNVLVVNPSAGDVIEGTGGVRKIRVAAKGHGKRGGARVIYYHFVSASQIVLLMIY
PKNEQQDLTAVERKSLKAAIEHWR

Nucleotide

• Length: 315 bp

>T178831 NZ_CP084322:c152433-151909 [Pseudomonas aeruginosa]
ATGAAAATCATCGGAGTGCAGGACCTCCCCTGCTACCGAATGCACACTCCCAAGTGGTCCGTTGCGCCTACATCTGGAGC
TGGAGCTGCAACTCAGGGTGGCCGGGTTAACCGAAAGGGGCTAGAGGCTCTATACCTCTCGCTTGAGTCGGCAACGGCCA
TTGCTGAGTTTCAGCAAGCAAGCGCATTCCTTCCTCCTGGTCTGCTGATCAGCTATTCCCTCAGTCTGGATCGAGTGATC
GACTTCACCGGCGGCTATAACGATACCTGGGCCCCGATCTGGCAAGACTTCTTCTGCGACTGGAGAAAGATTCGCTTCAA
CGACGGCGTTGAGCCGCCGAGCTGGATTATCGGAGATCTGGCTATCGAGGCTGACTGTGCTGGGATTCTGTTCGAGTCCG
TTGCGAACCCTGGAGGTCGGAACCTGGTGCTTTTCACCGATCAGTTGCCGGTGCATGGGAATATTGTGGTCAACGATCCT
CGCGGGGATCTCCCTACCGACCAGTCATCTTGGACGCGCCCGTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10828.41 Da
• Isoelectric Point: 7.3219

>AT178831 WP_003189536.1 NZ_CP063780:c2009738-2009451 [Pseudomonas sp. OST1909]
MSRFFDELMESVQEMNEILRGEREPSREFHVDSMRVKEIRKATGLTQAKFAALIDVQLGTLRNWEQGRREPTGPAKALLK
AIHNDPVHVLNALAS

Nucleotide

• Length: 288 bp

>AT178831 NZ_CP084322:c152825-152430 [Pseudomonas aeruginosa]
ATGGCCAGCAAAGCCGTATTAGAGCTTCGCCCCACCAACTTCGCGGGCTTGATGGACGCTCTCAAAGAGGTGAACCGTGT
GATGTTCTCTCCTGAGCGCTTCCTCAAGGTTTTTTCGATGGACCAGCAGACCTTGGCGCTCCGGGCCCATGTCCATCGCA
ACACTGTGCGCAACGCGCCTGAATCGGAAAAGGTCCAGGCCTACATTCGTGATTCAGTGAAGGTTCTGCGCGCAGTGACC
GACATGGGCACGGACGTGACGAATGCCATCTTCTGGTTTAAGAACGAGCCTCTTTCCACGTTCGGCTATAAGACCGCGGA
GGAAGTGGTCAGCGAAGGAAAAACCGAGCAGCTGATCGCTTTCCTTCAATCTTGGGAAGCTGGTGCCCAAGGATGA