Detailed information of TA system TA178820

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 22835..23077
• Replicon: plasmid pHK02-1
• Accession: NZ_CP063775
• Organism: Escherichia coli O25b:H4-ST131

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: INT78_RS24625
• Protein ID: WP_001312861.1
• Coordinates: 22835..22993 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	23037..23077 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INT78_RS24605	18082..18678	+	597	Protein_20	IS4-like element ISVsa5 family transposase	-
INT78_RS24610	18788..19594	+	807	Protein_21	IS4-like element ISVsa5 family transposase	-
INT78_RS24615	19646..20350	-	705	WP_001067858.1	IS6-like element IS26 family transposase	-
INT78_RS24620	20474..22087	+	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
INT78_RS24625	22835..22993	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	23037..23077	-	41	NuclAT_1	-	Antitoxin
-	23037..23077	-	41	NuclAT_1	-	Antitoxin
-	23037..23077	-	41	NuclAT_1	-	Antitoxin
-	23037..23077	-	41	NuclAT_1	-	Antitoxin
-	24521..24707	-	187	NuclAT_0	-	-
-	24521..24707	-	187	NuclAT_0	-	-
-	24521..24707	-	187	NuclAT_0	-	-
-	24521..24707	-	187	NuclAT_0	-	-
INT78_RS24635	24719..25438	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
INT78_RS24640	25435..25869	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
INT78_RS24645	25924..27882	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..58468	58468
	-	inside	IScluster/Tn	-	-	5563..24473	18910

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T178820 WP_001312861.1 NZ_CP063775:c22993-22835 [Escherichia coli O25b:H4-ST131]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T178820 NZ_CP084316:c2716323-2716066 [Salegentibacter mishustinae]
ATGAAATATACATTTGTTGACGAATCTTGGGAGGACTATTTATATTGGCAAAAAACGGATAAGAAAAAACTTAAAAAAAT
AAACGAACTTTTAAAGGATATTTCAAGAAATCCATTTGACGGAATCGGAAAACCAGAACCTTTAAAACATAAATATGCTG
GATTTTGGTCAAGAAGAATTGATAGTGAACATAGACTGATTTATCAATACAAAGAGGGGGAAATATTAATTGCAAAATGC
AGATTTCATTATGACTAA

Antitoxin

Nucleotide

• Length: 41 bp

>AT178820 NZ_CP063775:c23077-23037 [Escherichia coli O25b:H4-ST131]
TTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin