178815

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 4132551..4133230 Replicon chromosome
Accession NZ_CP063774
Organism Escherichia coli O25b:H4-ST131

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag INT78_RS19685 Protein ID WP_000057523.1
Coordinates 4132551..4132853 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag INT78_RS19690 Protein ID WP_000806442.1
Coordinates 4132889..4133230 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
INT78_RS19660 4127927..4129579 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
INT78_RS19665 4129617..4130120 - 504 WP_000667000.1 hypothetical protein -
INT78_RS19670 4130117..4130917 - 801 WP_000439798.1 hypothetical protein -
INT78_RS19675 4130941..4131420 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
INT78_RS19680 4131624..4132418 - 795 WP_000365147.1 TraB/GumN family protein -
INT78_RS19685 4132551..4132853 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
INT78_RS19690 4132889..4133230 + 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
INT78_RS19695 4133288..4135792 - 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
INT78_RS19700 4136054..4136986 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T178815 WP_000057523.1 NZ_CP063774:4132551-4132853 [Escherichia coli O25b:H4-ST131]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T178815 NZ_CP084312:1216402-1216695 [Bifidobacterium pseudolongum]
ATGACGCTTAAAACCACCAAATGGGATGTTACGGAATATCTTGATAATGAAGAGGATATCGCCGAATACCTCAACGCAGT
AATCGAATTGGACGATCCTGCGTTGCTCCAAGCTGCTATTGGTGATGTTGCCAAGGCGCAGGGGATGACGAAAATCGCCA
AAGATGCAGGCCTAGGCAGAGAAAGCCTATATAAAAGTCTGAGTGCACAAGGCAATCCTTCTTTCACGACCATATTGAAA
GTACTGCATGCTCTCGGTACAAGAATCAGCATTCAACCAGAACACGCTATCTGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT178815 WP_000806442.1 NZ_CP063774:4132889-4133230 [Escherichia coli O25b:H4-ST131]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT178815 NZ_CP084312:1216098-1216400 [Bifidobacterium pseudolongum]
ATGGAGATTCGTACTACTGAGCAATTCGATAAATGGTTCCGCAAGTTGCGGGACCGCAAAGCAAAAGCCAAAATCAGCGC
ACGGCTCAGACAATGCGAAATCTCCGACCATATCACTGGAGATCATCATGGCATCGGCGGTGGCATAAGTGAAATGAGAT
TCCATTTTGGACCGGGATATCGTATCTACTACACCGTTGACAATGATGTGCTTGTTATTTTACTGATCGGAAGTGGTAAA
GACGATCAGTCCAGAGGGATTGCCGAAGCGAGAAAACTTCTTGCTTTATATCGAGGAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
AlphaFold DB S1QAY3

References