Detailed information of TA system TA178802

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2886615..2887217
• Replicon: chromosome
• Accession: NZ_CP063774
• Organism: Escherichia coli O25b:H4-ST131

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: INT78_RS13765
• Protein ID: WP_000897302.1
• Coordinates: 2886615..2886926 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: INT78_RS13770
• Protein ID: WP_000356397.1
• Coordinates: 2886927..2887217 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INT78_RS13740	2882529..2883128	+	600	WP_001296610.1	glucose-1-phosphatase	-
INT78_RS13745	2883122..2883994	+	873	WP_000920762.1	virulence factor BrkB family protein	-
INT78_RS13750	2883991..2884428	+	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
INT78_RS13755	2884473..2885414	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
INT78_RS13760	2885478..2886386	-	909	WP_001385591.1	alpha/beta hydrolase	-
INT78_RS13765	2886615..2886926	+	312	WP_000897302.1	hypothetical protein	Toxin
INT78_RS13770	2886927..2887217	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
INT78_RS13775	2887576..2887854	+	279	WP_001296612.1	hypothetical protein	-
INT78_RS13780	2888251..2888469	+	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
INT78_RS13785	2888654..2889103	-	450	WP_032140890.1	hypothetical protein	-
INT78_RS13790	2889419..2890267	-	849	WP_001038650.1	hypothetical protein	-
INT78_RS13795	2890557..2890799	+	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
INT78_RS13800	2890981..2891910	-	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T178802 WP_000897302.1 NZ_CP063774:2886615-2886926 [Escherichia coli O25b:H4-ST131]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T178802 NZ_CP084300:433382-433768 [Acinetobacter pseudolwoffii]
ATGGATAATCGCTGTTTCAAGCTGAAACGCAGTTATTGTGCACTGGCGTTTCAGCTTTTTATTTTTGCACTGTTGATGTA
CCTCCTATACCAGCTATTGCCGATTGCGATTTGGCTGCTGTGTGCTGCGATTGGACTGGTGATTTATCAGCTGTTTTATC
GCAAGACGCCCCAGATTGAACAGTTTGAATATCTGGATGGACGTGAATGGTCATTGACGGCCAAATCTCGGCCCACCTGT
CGGGTATGTGTTAGTCATGTGATTGATCATCAAGCTTATATTGTGGTGTATTTTCAGCACGCCAAGGCTCAGCCTTTATT
GATCTGGTGTGACCAATTGCCGCTAAAACAATGGAAATCCCTGAAAGTTCTGACCAAACTTATCTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT178802 WP_000356397.1 NZ_CP063774:2886927-2887217 [Escherichia coli O25b:H4-ST131]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT178802 NZ_CP084300:433132-433389 [Acinetobacter pseudolwoffii]
ATGACTGATGACATCACCTTAGAAGAGCGCAAAGTGATTTATCGCGCGCGTCGTGGCCTAAAAGAGCTGGATGTGTATTT
TGATCCCTACGTAAAAAACTACTACTTAACTGCTGATCCATTTGAAAAGCAGATGTTTGCTGAGTTGGTCGATCAGGAAG
ATCCAGATCTGCTGGACTGGTTTATGGAAGTCTCTGAGCCGCCGCGTCCTGAATTAAAAGATTTGATCAAGAAGCTTAAA
TTTTACGTCCATGGATAA

Structures

Toxin

• AlphaFold DB: S1P416