178800

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2077389..2078188 Replicon chromosome
Accession NZ_CP063774
Organism Escherichia coli O25b:H4-ST131

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag INT78_RS09855 Protein ID WP_000347251.1
Coordinates 2077724..2078188 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag INT78_RS09850 Protein ID WP_001296435.1
Coordinates 2077389..2077724 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
INT78_RS09835 2073174..2073944 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
INT78_RS09840 2073960..2075294 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
INT78_RS09845 2075669..2077240 + 1572 WP_001273763.1 galactarate dehydratase -
INT78_RS09850 2077389..2077724 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
INT78_RS09855 2077724..2078188 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
INT78_RS09860 2078243..2079052 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
INT78_RS09865 2079301..2080581 + 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
INT78_RS09870 2080604..2081077 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
INT78_RS09875 2081088..2081867 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
INT78_RS09880 2081857..2082735 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
INT78_RS09885 2082753..2083187 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T178800 WP_000347251.1 NZ_CP063774:2077724-2078188 [Escherichia coli O25b:H4-ST131]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T178800 NZ_CP084299:c3522-3232 [Acinetobacter baumannii]
ATGGTTAAAATAGCTGATCTACCTAGCTTTGACATGGCAGAAGCACTTAAGAGTGAAGAAGATATTGTAATGTATCTTAA
TATGGTTCTTGAAGAGAATGACCCTGCTGAACTAGCTCATGCACTTGGTGTAATTGCTAAAGCTCGTGGTATGACTCAAA
TCGCTAAAGAAGCAGGAATTGGACGTGAAGCACTTTACAAAGCACTCCGTCAAGATTCAGCACCTCGTTTTGATACGATT
AATCGTGTAGTAAATGCTTTAGGTCTAAAACTAACTGTGCAGAATGCATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT178800 WP_001296435.1 NZ_CP063774:2077389-2077724 [Escherichia coli O25b:H4-ST131]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT178800 NZ_CP084299:c3822-3526 [Acinetobacter baumannii]
ATGATTGAAATTAAACGTCTACCAGAATTTGATGAATGGTTGGATGGCATTAAAGACAATATGACCCGCATTCGCTTGAA
TCGCCGATTAGATAAGGTTCAACGTGGGAACTGGGGAGACATTAAACCTCTCCAAGATGGTGTTTGGGAAATGAGAGAGT
TCTTCGGTTCAGGATGGAGGATGTATTACATCCAACATGGTGACGTAGTGATTGTAATGCTTGGAGGTGGTGATAAATCT
ACACAGCAAAAGGATATAGAACGAGCTGTTGATTTATCAAAATCTTTAGAGGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References