Detailed information of TA system TA178796

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 788064..788566
• Replicon: chromosome
• Accession: NZ_CP063774
• Organism: Escherichia coli O25b:H4-ST131

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: INT78_RS03960
• Protein ID: WP_000767819.1
• Coordinates: 788064..788318 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: INT78_RS03965
• Protein ID: WP_001259253.1
• Coordinates: 788315..788566 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INT78_RS03935	783882..784340	-	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-
INT78_RS03940	784557..785915	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
INT78_RS03945	785905..785967	-	63	WP_010723108.1	membrane protein YoeI	-
INT78_RS03950	786182..787111	-	930	WP_000803366.1	LysR family transcriptional regulator	-
INT78_RS03955	787157..787981	-	825	WP_000754737.1	SDR family oxidoreductase	-
INT78_RS03960	788064..788318	-	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
INT78_RS03965	788315..788566	-	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
INT78_RS03970	788849..788899	+	51	WP_001364200.1	his operon leader peptide	-
INT78_RS03975	789045..789944	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
INT78_RS03980	789950..791254	+	1305	WP_001296211.1	histidinol dehydrogenase	-
INT78_RS03985	791251..792321	+	1071	WP_000108967.1	histidinol-phosphate transaminase	-
INT78_RS03990	792321..793388	+	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	783882..784340	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T178796 WP_000767819.1 NZ_CP063774:c788318-788064 [Escherichia coli O25b:H4-ST131]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T178796 NZ_CP084296:4145163-4145531 [Providencia alcalifaciens]
ATGGTAAAAGCGAAAAGGCCTCACAAAGGCCAGATTTGGCACATTAATGGCGATCCTGTCGCAGGTAAGGAATTCAAGGG
ACCCCACTACTATTTAGTGGTTACAGATGCACAAATCAACACCGCACTTGGTACGTCAATTTGCGTTCCTATTACAAGTG
GAGGGAGCCGCGCCAGAACTCAAAATGTCACTGTTTACTTAGATGGTTCAAGTACAGATACAGGTAACATTACTGGCTGT
ATCTTATGTTATCAACTCCGCTCGTTAGATCTAATAGCCAGAAAAGCCACTTACTCAGCAACACTAAATGATGATATTTT
CGAAGAGGTTCTCAGCAATATTATTGATATCATTGACCCTCAAATTTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT178796 WP_001259253.1 NZ_CP063774:c788566-788315 [Escherichia coli O25b:H4-ST131]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT178796 NZ_CP084296:4144900-4145163 [Providencia alcalifaciens]
ATGTCAACAACCAAATTAAGGCAGCAAGGCGGAGCTGTTGTTTTAACTATCCCAATTGAAGTCGCCAATATGGCAGGATG
GGTTGTCGGTATGAAATTAAATATTGATGCTGTTGGAGAATCCATTTGCATAAAACCGGTCAAACGCGTGGCTAGAGGAC
GAAAATCTATCAATGAGCTGCTATCAGGTATTGATAAAGAAGAGATTAAGCTATTAAACAGTGAAGTCGATGATTCTGAC
CCAGAGGGAAATGAGGTTATTTAA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3