Detailed information of TA system TA178778

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1903774..1904276
• Replicon: chromosome
• Accession: NZ_CP063773
• Organism: Pectobacterium carotovorum subsp. carotovorum PCCS1

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A7W2E5L4
• Locus tag: IQ281_RS08635
• Protein ID: WP_039282578.1
• Coordinates: 1903774..1904028 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: IQ281_RS08640
• Protein ID: WP_194431517.1
• Coordinates: 1904025..1904276 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQ281_RS08615	1898911..1900173	-	1263	WP_181845053.1	L-rhamnose isomerase	-
IQ281_RS08620	1900170..1901660	-	1491	WP_194431514.1	rhamnulokinase	-
IQ281_RS08625	1901996..1902835	+	840	WP_194431515.1	HTH-type transcriptional activator RhaS	-
IQ281_RS08630	1902860..1903714	+	855	WP_194431516.1	helix-turn-helix domain-containing protein	-
IQ281_RS08635	1903774..1904028	-	255	WP_039282578.1	Txe/YoeB family addiction module toxin	Toxin
IQ281_RS08640	1904025..1904276	-	252	WP_194431517.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
IQ281_RS08645	1904352..1906061	-	1710	WP_194431518.1	ABC transporter ATP-binding protein	-
IQ281_RS08650	1906058..1906924	-	867	WP_010285293.1	ABC transporter permease	-
IQ281_RS08655	1906917..1907876	-	960	WP_012773138.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10229.79 Da
• Isoelectric Point: 8.0353

>T178778 WP_039282578.1 NZ_CP063773:c1904028-1903774 [Pectobacterium carotovorum subsp. carotovorum PCCS1]
VKLIWSEEAWEDYLYWQDIDKRMVKKINELIKETRRTPFEGKGKPEPLKHNLAGFWSRRITEEHRLVYAITDDAMMIAAC
RYHY

Nucleotide

• Length: 255 bp

>T178778 NZ_CP084290:c804230-803853 [Citrobacter werkmanii]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9383.64 Da
• Isoelectric Point: 4.6119

>AT178778 WP_194431517.1 NZ_CP063773:c1904276-1904025 [Pectobacterium carotovorum subsp. carotovorum PCCS1]
MRTISYSEARQNLSATMMKTVEDRAPILITRQNGESCVLMSLEEYNSLEETAYLLRSPANAKRLMNSIESLKAGNGEERD
IIE

Nucleotide

• Length: 252 bp

>AT178778 NZ_CP084290:c804646-804320 [Citrobacter werkmanii]
ATGAGTAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATAAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCAACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCTTCGGCTCCTGTGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAA
AAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7W2E5L4