Detailed information of TA system TA178767

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 6914..7565
• Replicon: plasmid unnamed2
• Accession: NZ_CP063771
• Organism: Acinetobacter sp. Ac-14

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A062G190
• Locus tag: IOD65_RS19485
• Protein ID: WP_005065055.1
• Coordinates: 6914..7258 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IOD65_RS19490
• Protein ID: WP_005065057.1
• Coordinates: 7263..7565 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOD65_RS19465	2777..3490	+	714	WP_005065071.1	hypothetical protein	-
IOD65_RS19470	4188..5117	+	930	WP_005065049.1	replication initiation protein RepM	-
IOD65_RS19475	5196..5726	+	531	WP_005065050.1	plasmid replication DNA-binding protein	-
IOD65_RS19480	6076..6393	+	318	WP_005065053.1	hypothetical protein	-
IOD65_RS19485	6914..7258	+	345	WP_005065055.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IOD65_RS19490	7263..7565	+	303	WP_005065057.1	XRE family transcriptional regulator	Antitoxin
IOD65_RS19495	7657..8058	+	402	WP_005065059.1	hypothetical protein	-
IOD65_RS19500	8110..8784	+	675	WP_005065062.1	HEPN domain-containing protein	-
IOD65_RS19505	8875..9186	-	312	WP_005065064.1	BrnA antitoxin family protein	-
IOD65_RS19510	9173..9460	-	288	WP_002124950.1	BrnT family toxin	-
IOD65_RS19515	9973..11460	+	1488	WP_005065066.1	SulP family inorganic anion transporter	-
IOD65_RS19520	11473..12324	+	852	WP_005065068.1	universal stress protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..17334	17334

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13143.23 Da
• Isoelectric Point: 6.4651

>T178767 WP_005065055.1 NZ_CP063771:6914-7258 [Acinetobacter sp. Ac-14]
MWTVITTDLFNEWLEQQDEITQEKVLAALVVLQLQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSGKKRFYTEMLAIADKQYELHLSTLGE

Nucleotide

• Length: 345 bp

>T178767 NZ_CP084265:2893980-2894327 [Caballeronia sp. Lep1P3]
ATGCTAACATCCGATACTGGCCCGTGGACCATCGCTTATTACAGCGCGCGCGTCAAAGATGAAGTCTGGCAACTGCCTCC
GGGCATCCTCGCCAGTTACTTTCGCCTCACTCGCCCGCTAAGGGAGCACGGTCCCGGCCTGCCTCTTCCTCATGCGCGGC
CGATGGGCGACAAACTTTTTGAACTGCGTCCGGCAGGTCACGACGGCATTGGACGCGTGTTCTATTGCACGCTCGAGGGA
AGGCGCATCGTCATATTGCATTCCTTCGCCAAGAAGACGCAGCGCACGCCGCTGAAGGAACTGCATCTTGCCCGCAAACG
GTTGAAGGAGGTTCGACGAAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11280.08 Da
• Isoelectric Point: 5.8961

>AT178767 WP_005065057.1 NZ_CP063771:7263-7565 [Acinetobacter sp. Ac-14]
MAKTLQELLANRSPESQARIQKMADELLLENQLYLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT178767 NZ_CP084265:2894320-2894655 [Caballeronia sp. Lep1P3]
ATGGCTAAGACCCGCTGGCAGCAGCAAATGGAAGCGCGCTACGATCCCGTGCCGCATTCGGACGAGGAAGTCGCGCAGAT
GTTCGCCGCCGATCCCGCGTTGAAGGCCGCCTACGACGCGCTCGAAGACGAATACGCCGCACTCGATGCTCTGCTTCGCG
CGCGCAAGGAAGCCGGCCTGACGCAGGCGCAAGTCGCTGAACGCATGGGCACGACCGCGTCAGCCGTGGCGCGGCTCGAA
GGATCGCTCGTCAGCGAAAAGCATTCGCCGACGCTGGCCACGCTCCGCAAATACGCCACCGCTTGCGGCAAGACCTTGAG
AATCTCGTTCGTCTGA

Structures

Toxin

• AlphaFold DB: A0A062G190