Detailed information of TA system TA178766

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 74394..74856
• Replicon: plasmid unnamed1
• Accession: NZ_CP063770
• Organism: Acinetobacter sp. Ac-14

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3R9PS35
• Locus tag: IOD65_RS19260
• Protein ID: WP_000033474.1
• Coordinates: 74394..74582 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IOD65_RS19265
• Protein ID: WP_001258992.1
• Coordinates: 74569..74856 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOD65_RS19220	69960..70277	+	318	WP_000900860.1	hypothetical protein	-
IOD65_RS19225	70802..71158	+	357	WP_000269903.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IOD65_RS19230	71151..71453	+	303	WP_001140619.1	XRE family transcriptional regulator	-
IOD65_RS19235	71446..71655	+	210	WP_000069471.1	hypothetical protein	-
IOD65_RS19240	71765..71992	-	228	WP_005071544.1	hypothetical protein	-
IOD65_RS19245	72750..73295	-	546	Protein_85	IS5 family transposase	-
IOD65_RS19250	73223..73611	-	389	Protein_86	AAA family ATPase	-
IOD65_RS19255	73797..74117	+	321	WP_002123990.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IOD65_RS19260	74394..74582	+	189	WP_000033474.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IOD65_RS19265	74569..74856	+	288	WP_001258992.1	helix-turn-helix transcriptional regulator	Antitoxin
IOD65_RS19270	75140..75364	-	225	WP_005071551.1	hypothetical protein	-
IOD65_RS19275	75388..75945	-	558	Protein_91	hypothetical protein	-
IOD65_RS19280	75985..77301	-	1317	WP_005071556.1	hypothetical protein	-
IOD65_RS19285	77285..77826	-	542	Protein_93	DUF2280 domain-containing protein	-
IOD65_RS19290	77886..78527	-	642	WP_005071561.1	hypothetical protein	-
IOD65_RS19295	78496..78963	-	468	WP_005071563.1	hypothetical protein	-
IOD65_RS19300	79024..79479	-	456	WP_005071565.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..117968	117968
	-	flank	IS/Tn	-	-	72750..72995	245

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (2-55)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-90)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7137.56 Da
• Isoelectric Point: 10.9048

>T178766 WP_000033474.1 NZ_CP063770:74394-74582 [Acinetobacter sp. Ac-14]
MSKGLFELRLKSQEGIARVMYCTLVGKRIVMLHSFVKKTQKTPKQDLNLALDRMKEVKNANT

Nucleotide

• Length: 189 bp

>T178766 NZ_CP084265:c2146871-2146671 [Caballeronia sp. Lep1P3]
ATGGATTCATCGCGGCTGATACGAATGCTTCTGGACGATGGTTGGAAGCTCGCTCGCATTACTGGCAGTCATCATCATTT
CAAACGCGCGGGAAGGGGCGTTCTAGTGACGGTGCCCCATCCGAGGAAGGATCTCTCGATCGGGCTGGTGCGAGCCATCC
TCAAAGCTGCCGGACTGCGCGACCGGCTGTACGCTTCGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10563.32 Da
• Isoelectric Point: 9.7308

>AT178766 WP_001258992.1 NZ_CP063770:74569-74856 [Acinetobacter sp. Ac-14]
MRTHEEMKALALSRSAVRTEYERIEREEMPLLDMVLTALREAGLSQAQIAERMGTNVPAVSRLEKALITGKPSPSIATLQ
KYAAAIGKHVEVRFV

Nucleotide

• Length: 288 bp

>AT178766 NZ_CP084265:c2146657-2146256 [Caballeronia sp. Lep1P3]
TTGGAATTCGCTGTCGCAATCCTGAAGGACCGGGAAAGCTCCTATGGAGTGATCGTCCCGGACATTCCCGGCGTCTTTTC
AGCCGGAGACACGGTCGACGAGGCAATCAAGAACACCAGGGAAGCGATCTACGGACACGTCTCGGTCCTGATCGAAGAGG
GCATGGAGGTGCCTCTCGCCGCTTCGCCGATCGACGAACTCGTCGCTAACCCCGACTATGCAGGCGCAATCTGGGCGTTC
GTGGAAGTCGATCTTTCGAAGCTCGACTCGAAGCCCGAACGCATTAACATCAGCCTGCCGCGCTTCGTCCTTCGCAAGAT
CGACAGCTACGTCGAGGCGCGCCACGAAACGCGCAGCGGCTTTCTCGCGCGAGTCGCGCTCGAAGCCATTGCCAATGGCT
GA

Structures

Toxin

• AlphaFold DB: A0A3R9PS35