Detailed information of TA system TA178764

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 2798..3383
• Replicon: plasmid unnamed1
• Accession: NZ_CP063770
• Organism: Acinetobacter sp. Ac-14

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7S7BY50
• Locus tag: IOD65_RS18835
• Protein ID: WP_000897311.1
• Coordinates: 2798..3118 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IOD65_RS18840
• Protein ID: WP_000369784.1
• Coordinates: 3111..3383 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOD65_RS18825	696..1133	-	438	Protein_1	IS3 family transposase	-
IOD65_RS18830	1187..2119	+	933	WP_000743272.1	IS5-like element IS17 family transposase	-
IOD65_RS18835	2798..3118	+	321	WP_000897311.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IOD65_RS18840	3111..3383	+	273	WP_000369784.1	helix-turn-helix domain-containing protein	Antitoxin
IOD65_RS18845	3416..4216	+	801	WP_000604996.1	DUF2971 domain-containing protein	-
IOD65_RS18850	4307..4608	+	302	Protein_6	transposase	-
IOD65_RS18855	4724..4936	+	213	WP_001123161.1	hypothetical protein	-
IOD65_RS18860	5262..6119	-	858	WP_000609033.1	LysR family transcriptional regulator	-
IOD65_RS18865	6116..7123	-	1008	WP_005071472.1	2-hydroxyacid dehydrogenase	-
IOD65_RS18870	7165..7962	-	798	WP_004757464.1	phosphonate ABC transporter, permease protein PhnE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..117968	117968
	-	inside	IScluster/Tn	-	-	696..4642	3946

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-84)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12214.03 Da
• Isoelectric Point: 7.9500

>T178764 WP_000897311.1 NZ_CP063770:2798-3118 [Acinetobacter sp. Ac-14]
MLFIETSIFTKQIKELVTDEEYRQLQQDLLIQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETSILRQLVKEQFNG

Nucleotide

• Length: 321 bp

>T178764 NZ_CP084254:c16541-16140 [Caballeronia sp. Sq4a]
ATGCCGCGCTTCATGCTGGACACCAATATGTGCATCTACCTAATGAAGAACCAGCCCGAGCAGGTGGCGAAACGCTTCGC
GGAGTGCTTTGTCGGCGACGTGGTCATGTCAGCTATCACGTATGCAGAGTTGGAATACGGTGTCGCCGTTTGTGCAAATC
CGGCGAAAGAGCGCCGCACCCTCGCCGCGTTGATTGAGGACATCCCGGTGGCTCCGTTCGATGCTGCGGCGGCGGTGGCT
TATGGCCCGATTCGCGAAGCAACTCGCGAGCGCAAGAAGGACCACCTAGATAAGCTGATCGCCGCACATGCGGTCGCGCT
CGACGTTGCCCTCGTCACGAACAACACAAAAGACTTTGTCAGTTATCCGGGCGTCAAGCTCGAGAACTGGCTGAGCGATT
GA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10145.57 Da
• Isoelectric Point: 7.0146

>AT178764 WP_000369784.1 NZ_CP063770:3111-3383 [Acinetobacter sp. Ac-14]
MDNNLFDDLVTSIKEAGAIKRNEIKASRVTELELPDIKEVREKTGLTQAEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDTNPTLI

Nucleotide

• Length: 273 bp

>AT178764 NZ_CP084254:c16777-16541 [Caballeronia sp. Sq4a]
GTGCAAATCACACGAGTTTTCAGAAACGGCAACTCCCAGGCCGTCCGCATCCCTGCTGACCTCGCCTATGAGCGCAGCGA
CCTTGAGCTTGAGATCGAACGCGTGGGCGATGAAATCCGCATCCGACCGGCGCGCCGACCGCTCGCCGGTGTGTTGAAGA
AATTCGCCAAGTTCAGCCCCGACTTTATGGCCCAGGGACGAGGCGAGCAGGAACAAGCAGAACGCGAGGGCCTGTAA

Structures

Toxin

• AlphaFold DB: A0A7S7BY50