Detailed information of TA system TA178756

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 106458..106711
• Replicon: plasmid pFRPDR_KPC
• Accession: NZ_CP063761
• Organism: Klebsiella pneumoniae strain FRPDR

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: IM727_RS29330
• Protein ID: WP_001312851.1
• Coordinates: 106562..106711 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	106458..106517 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IM727_RS29295	102236..102940	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
IM727_RS29300	103004..103165	+	162	Protein_125	DNA helicase	-
IM727_RS29305	103185..103931	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
IM727_RS29310	103986..104546	+	561	WP_000139328.1	fertility inhibition protein FinO	-
IM727_RS29315	104678..104878	+	201	WP_015059022.1	hypothetical protein	-
IM727_RS29320	105264..105863	+	600	WP_032083981.1	hypothetical protein	-
IM727_RS29325	106027..106257	+	231	WP_001736714.1	hypothetical protein	-
-	106458..106517	-	60	NuclAT_1	-	Antitoxin
-	106458..106517	-	60	NuclAT_1	-	Antitoxin
-	106458..106517	-	60	NuclAT_1	-	Antitoxin
-	106458..106517	-	60	NuclAT_1	-	Antitoxin
IM727_RS29330	106562..106711	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
IM727_RS29335	106995..107243	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..107554	107554

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T178756 WP_001312851.1 NZ_CP063761:106562-106711 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T178756 NZ_CP084242:99957-100439 [Klebsiella pneumoniae]
GTGGGATGTATAACTGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGGGAAACGGTACTCGA
TGAATGGTTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCACGAACTTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTTTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCGCAAACTTATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT178756 NZ_CP063761:c106517-106458 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin