Detailed information of TA system TA178753

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 36382..36651
• Replicon: plasmid pFRPDR_KPC
• Accession: NZ_CP063761
• Organism: Klebsiella pneumoniae strain FRPDR

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: IM727_RS28905
• Protein ID: WP_001312861.1
• Coordinates: 36493..36651 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	36382..36447 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IM727_RS28880	32092..32619	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
IM727_RS28885	32677..32910	+	234	WP_000006003.1	DUF905 family protein	-
IM727_RS28890	32971..34994	+	2024	Protein_43	ParB/RepB/Spo0J family partition protein	-
IM727_RS28895	35063..35497	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
IM727_RS28900	35494..36213	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	36225..36449	+	225	NuclAT_0	-	-
-	36225..36449	+	225	NuclAT_0	-	-
-	36225..36449	+	225	NuclAT_0	-	-
-	36225..36449	+	225	NuclAT_0	-	-
-	36382..36447	-	66	-	-	Antitoxin
IM727_RS28905	36493..36651	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
IM727_RS28910	36889..37266	-	378	Protein_47	hypothetical protein	-
IM727_RS28915	37566..37862	+	297	WP_001272251.1	hypothetical protein	-
IM727_RS28920	37973..38794	+	822	WP_001234445.1	DUF945 domain-containing protein	-
IM727_RS28925	39091..39738	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
IM727_RS28930	40015..40398	+	384	WP_000124981.1	relaxosome protein TraM	-
IM727_RS28935	40589..41275	+	687	WP_015059009.1	PAS domain-containing protein	-
IM727_RS28940	41369..41596	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..107554	107554

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T178753 WP_001312861.1 NZ_CP063761:36493-36651 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T178753 NZ_CP084241:c4592921-4592388 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGTATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT178753 NZ_CP063761:c36447-36382 [Klebsiella pneumoniae]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin