Detailed information of TA system TA178697

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): holin-SprF3/-
• Location: 18940..19388
• Replicon: chromosome
• Accession: NZ_CP063745
• Organism: Staphylococcus aureus strain NAS_AN_212

Toxin (Protein)

• Gene name: holin
• Uniprot ID: A0A853P973
• Locus tag: IF732_RS00115
• Protein ID: WP_000387656.1
• Coordinates: 19086..19388 (+)
• Length: 101 a.a.

Antitoxin (RNA)

Gene name	SprF3
Locus tag	-
Coordinates	18940..19006 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF732_RS00085 (14041)	14041..17721	+	3681	WP_000582140.1	phage tail spike protein	-
IF732_RS00090 (17708)	17708..17860	+	153	WP_001181555.1	hypothetical protein	-
IF732_RS00095 (17907)	17907..18194	+	288	WP_001040257.1	hypothetical protein	-
IF732_RS00100 (18252)	18252..18548	+	297	WP_000539688.1	DUF2951 domain-containing protein	-
IF732_RS00105 (18780)	18780..18887	+	108	WP_000253688.1	putative holin-like toxin	-
- (18940)	18940..19006	-	67	NuclAT_0	-	Antitoxin
- (18940)	18940..19006	-	67	NuclAT_0	-	Antitoxin
- (18940)	18940..19006	-	67	NuclAT_0	-	Antitoxin
- (18940)	18940..19006	-	67	NuclAT_0	-	Antitoxin
IF732_RS00110 (18931)	18931..19035	-	105	Protein_21	hypothetical protein	-
IF732_RS00115 (19086)	19086..19388	+	303	WP_000387656.1	phage holin	Toxin
IF732_RS00120 (19400)	19400..20854	+	1455	WP_240104857.1	N-acetylmuramoyl-L-alanine amidase	-
IF732_RS00125 (20949)	20949..21398	-	450	WP_000727649.1	chemotaxis-inhibiting protein CHIPS	-
IF732_RS00130 (22083)	22083..22433	+	351	WP_000702263.1	complement inhibitor SCIN-A	-
IF732_RS00135 (22486)	22486..22746	-	261	WP_001791826.1	hypothetical protein	-
IF732_RS00140 (23057)	23057..23236	-	180	WP_000669789.1	hypothetical protein	-
IF732_RS00145 (23608)	23608..23805	-	198	WP_000239614.1	phospholipase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	chp / scn	1..22433	22432

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Holin (3-68)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11177.94 Da
• Isoelectric Point: 9.6587

>T178697 WP_000387656.1 NZ_CP063745:19086-19388 [Staphylococcus aureus]
MEAKVMARYVVLILALVNQFLANKGISPIPVDEESISSIILTVIALYTTYKDNPTSQEGRWANQKLKKYKAESKYRKATG
QAPIKEVMTPTNMNDTNDLG

Nucleotide

• Length: 303 bp

>T178697 NZ_CP084209:2609242-2609349 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Nucleotide

• Length: 67 bp

>AT178697 NZ_CP063745:c19006-18940 [Staphylococcus aureus]
ATAAATAGAAAAAGGGCAACATGCGGAAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGGCT

Structures

Toxin

• AlphaFold DB: A0A853P973

Antitoxin