Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 80..726 Replicon plasmid pEH01-18-02_3
Accession NZ_CP063521
Organism Escherichia coli strain EH01-18-02

Toxin (Protein)


Gene name higB Uniprot ID F4T8Q4
Locus tag IQS16_RS23255 Protein ID WP_000269926.1
Coordinates 80..430 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID F4T8Q5
Locus tag IQS16_RS23260 Protein ID WP_001259443.1
Coordinates 427..726 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS16_RS23255 80..430 + 351 WP_000269926.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IQS16_RS23260 427..726 + 300 WP_001259443.1 XRE family transcriptional regulator Antitoxin
IQS16_RS23265 757..1002 - 246 WP_001193619.1 hypothetical protein -
IQS16_RS23270 992..1261 - 270 WP_096950010.1 hypothetical protein -
IQS16_RS23275 1285..1827 - 543 WP_096950009.1 hypothetical protein -
IQS16_RS23280 1955..2488 - 534 WP_096950008.1 J domain-containing protein -
IQS16_RS23285 2857..3156 - 300 WP_096950007.1 hypothetical protein -
IQS16_RS23290 3230..3502 - 273 WP_096950006.1 hypothetical protein -
IQS16_RS23295 4452..5177 - 726 WP_096950005.1 RepA protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..24093 24093


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-105)

Antitoxin

Cro/C1-type helix-turn-helix domain

(32-80)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13742.77 Da        Isoelectric Point: 7.4217

>T178677 WP_000269926.1 NZ_CP063521:80-430 [Escherichia coli]
MWTVYFGRLFDEWFEEQELALKRKVLAELKHLEEFGPSLSRPHADTVKGSRHKNMKELRIQYEGHPIRAFFAFDPIRQAI
ILCAGDKSNDKKFYDRMIRIADEEFSTHLAELEGKK

Download         Length: 351 bp

>T178677 NZ_CP084207:c4398075-4397677 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10917.70 Da        Isoelectric Point: 4.6283

>AT178677 WP_001259443.1 NZ_CP063521:427-726 [Escherichia coli]
MRTLEEVIAAQSPESQAIIKAMADEMILETGLQMMREELQLSQKQVADAMGISQPAVTQLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRVAFNI

Download         Length: 300 bp

>AT178677 NZ_CP084207:c4398371-4398078 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A743U745

Antitoxin

Source ID Structure
AlphaFold DB A0A743U677

References