Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4063018..4063817 Replicon chromosome
Accession NZ_CP063518
Organism Escherichia coli strain EH01-18-02

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag IQS16_RS19435 Protein ID WP_000347266.1
Coordinates 4063353..4063817 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag IQS16_RS19430 Protein ID WP_001307405.1
Coordinates 4063018..4063353 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS16_RS19415 4058803..4059573 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
IQS16_RS19420 4059589..4060923 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
IQS16_RS19425 4061298..4062869 + 1572 WP_001273741.1 galactarate dehydratase -
IQS16_RS19430 4063018..4063353 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
IQS16_RS19435 4063353..4063817 + 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
IQS16_RS19440 4063872..4064681 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
IQS16_RS19445 4064930..4066210 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
IQS16_RS19450 4066233..4066706 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
IQS16_RS19455 4066717..4067496 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
IQS16_RS19460 4067486..4068364 + 879 WP_001315856.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
IQS16_RS19465 4068382..4068816 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4052362..4063817 11455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T178673 WP_000347266.1 NZ_CP063518:4063353-4063817 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T178673 NZ_CP084207:3418369-3418476 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT178673 WP_001307405.1 NZ_CP063518:4063018-4063353 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT178673 NZ_CP084207:c3418322-3418256 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References