Detailed information of TA system TA178649

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 40839..41441
• Replicon: chromosome
• Accession: NZ_CP063518
• Organism: Escherichia coli strain EH01-18-02

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: IQS16_RS00190
• Protein ID: WP_000897305.1
• Coordinates: 40839..41150 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IQS16_RS00195
• Protein ID: WP_000356397.1
• Coordinates: 41151..41441 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS16_RS00165	36753..37352	+	600	WP_001295269.1	glucose-1-phosphatase	-
IQS16_RS00170	37346..38218	+	873	WP_000920762.1	virulence factor BrkB family protein	-
IQS16_RS00175	38215..38652	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
IQS16_RS00180	38697..39638	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
IQS16_RS00185	39702..40610	-	909	WP_001162704.1	alpha/beta hydrolase	-
IQS16_RS00190	40839..41150	+	312	WP_000897305.1	hypothetical protein	Toxin
IQS16_RS00195	41151..41441	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
IQS16_RS00200	42027..42245	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
IQS16_RS00205	42464..42706	+	243	WP_001087409.1	hypothetical protein	-
IQS16_RS00210	43036..43965	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
IQS16_RS00215	43962..44597	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
IQS16_RS00220	44594..45496	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T178649 WP_000897305.1 NZ_CP063518:40839-41150 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T178649 NZ_CP084205:c4034020-4033670 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT178649 WP_000356397.1 NZ_CP063518:41151-41441 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT178649 NZ_CP084205:c4034265-4034014 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGACGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7