Detailed information of TA system TA178639

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 128093..128335
• Replicon: plasmid pEH01-18-04-A_1
• Accession: NZ_CP063516
• Organism: Escherichia coli strain EH01-18-04-A

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: IQS17_RS25275
• Protein ID: WP_001312861.1
• Coordinates: 128177..128335 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	128093..128133 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS17_RS25255	123289..125247	+	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-
IQS17_RS25260	125302..125736	+	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
IQS17_RS25265	125733..126452	+	720	WP_001276232.1	plasmid SOS inhibition protein A	-
-	126464..126650	+	187	NuclAT_0	-	-
-	126464..126650	+	187	NuclAT_0	-	-
-	126464..126650	+	187	NuclAT_0	-	-
-	126464..126650	+	187	NuclAT_0	-	-
IQS17_RS25270	126698..128066	+	1369	Protein_143	IS3-like element IS150 family transposase	-
-	128093..128133	+	41	NuclAT_1	-	Antitoxin
-	128093..128133	+	41	NuclAT_1	-	Antitoxin
-	128093..128133	+	41	NuclAT_1	-	Antitoxin
-	128093..128133	+	41	NuclAT_1	-	Antitoxin
IQS17_RS25275	128177..128335	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
IQS17_RS25280	128778..129113	+	336	WP_013023876.1	hypothetical protein	-
IQS17_RS25285	129026..129232	+	207	WP_000275859.1	hypothetical protein	-
IQS17_RS25290	129257..129544	+	288	WP_000107535.1	hypothetical protein	-
IQS17_RS25295	129663..130484	+	822	WP_001234469.1	DUF945 domain-containing protein	-
IQS17_RS25300	130781..131383	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
IQS17_RS25305	131714..132097	+	384	WP_001151566.1	relaxosome protein TraM	-
IQS17_RS25310	132231..132908	+	678	WP_001348626.1	PAS domain-containing protein	-
IQS17_RS25315	132996..133223	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / dfrA17	senB	1..149434	149434

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T178639 WP_001312861.1 NZ_CP063516:128177-128335 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T178639 NZ_CP084205:c2370174-2369998 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Nucleotide

• Length: 41 bp

>AT178639 NZ_CP063516:128093-128133 [Escherichia coli]
TTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin