Detailed information of TA system TA178633

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 20506..20745
• Replicon: plasmid pEH01-18-04-A_1
• Accession: NZ_CP063516
• Organism: Escherichia coli strain EH01-18-04-A

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: IQS17_RS24645
• Protein ID: WP_023144756.1
• Coordinates: 20611..20745 (+)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	20506..20566 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS17_RS24615	16299..16859	+	561	WP_000139341.1	fertility inhibition protein FinO	-
IQS17_RS24620	16990..17202	+	213	WP_013023861.1	hypothetical protein	-
IQS17_RS24625	17761..18186	+	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
IQS17_RS24630	18183..18533	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
IQS17_RS24635	18563..20175	+	1613	Protein_16	IS66-like element ISEc23 family transposase	-
IQS17_RS24640	20253..20539	+	287	Protein_17	DUF2726 domain-containing protein	-
-	20506..20566	-	61	NuclAT_2	-	Antitoxin
-	20506..20566	-	61	NuclAT_2	-	Antitoxin
-	20506..20566	-	61	NuclAT_2	-	Antitoxin
-	20506..20566	-	61	NuclAT_2	-	Antitoxin
IQS17_RS24645	20611..20745	+	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
IQS17_RS24650	21042..21296	+	255	WP_000083850.1	replication regulatory protein RepA	-
IQS17_RS24655	21402..21536	+	135	Protein_20	protein CopA/IncA	-
IQS17_RS24660	21533..21607	+	75	WP_031943482.1	RepA leader peptide Tap	-
IQS17_RS24665	21600..22457	+	858	WP_001617855.1	incFII family plasmid replication initiator RepA	-
IQS17_RS24670	23397..24050	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
IQS17_RS24675	24143..24400	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
IQS17_RS24680	24333..24734	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / dfrA17	senB	1..149434	149434

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T178633 WP_023144756.1 NZ_CP063516:20611-20745 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T178633 NZ_CP084205:1790523-1790774 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT178633 NZ_CP063516:c20566-20506 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin