Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2315851..2316650 Replicon chromosome
Accession NZ_CP063515
Organism Escherichia coli strain EH01-18-04-A

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag IQS17_RS11345 Protein ID WP_000347251.1
Coordinates 2316186..2316650 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag IQS17_RS11340 Protein ID WP_001296435.1
Coordinates 2315851..2316186 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS17_RS11325 2311636..2312406 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
IQS17_RS11330 2312422..2313756 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
IQS17_RS11335 2314131..2315702 + 1572 WP_001273763.1 galactarate dehydratase -
IQS17_RS11340 2315851..2316186 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
IQS17_RS11345 2316186..2316650 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
IQS17_RS11350 2316705..2317514 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
IQS17_RS11355 2317763..2319043 + 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
IQS17_RS11360 2319066..2319539 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
IQS17_RS11365 2319550..2320329 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
IQS17_RS11370 2320319..2321197 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
IQS17_RS11375 2321215..2321649 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T178618 WP_000347251.1 NZ_CP063515:2316186-2316650 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T178618 NZ_CP084197:c3851895-3851542 [Pantoea agglomerans]
ATGTGGGATGTGGATACAACCAAAAGATTTGATGAATGGTTTAAGGCTCAGACTGAGGAACTGAAGGAGGATATGCTCGC
CGCGATAGTTATCCTGTCTGAGTATGGGCCGCATTTAGCGAGGCCGTTTGCAGATACTGTAGACGGCTCTGATTTTTCGA
ATATGAAAGAATTAAGAGTGCAGCATCAAGGCAAACCGATACGGGCCTTCTTTGCTTTTGATCCTTCTCGCCGTGGCATT
GTGTTGTGTGCAGGCGATAAAACCGGGGTTAAGGAAAAGAAATTCTATAAAGACATGATCAAATTGGCAGATGCTGAATT
CCGCAAATATTTGAATGGAGGGGATAATGGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT178618 WP_001296435.1 NZ_CP063515:2315851-2316186 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT178618 NZ_CP084197:c3851549-3851250 [Pantoea agglomerans]
ATGGCTAATTTAAAAGAGCTGATGGCAAATCAAAGTCCGGAGAGTCGCGAACGCATTGCCAAAAAAGTCGATGCAATGCG
GCAGGTTATCGCGCTACACATGTTGCGTGAAGAACTTAACCTGTCACAGACAGAGATGGCGCACGCAATGGGTGTGAAAC
AGCCAACTATCGCCAGAATGGAACAGGCTGATAATGATCCTCGCCTTTCTACGCTGAAGCGTTACGTTACGGCTCTGGGA
GGCGAGATAAGTATCGATGTCAAATTGCCGACAGGCAAAAGAATAGCTTTTGATCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References