Detailed information of TA system TA178584

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2947193..2947695
• Replicon: chromosome
• Accession: NZ_CP063511
• Organism: Escherichia coli strain EH08-18-36

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: IQS18_RS14195
• Protein ID: WP_000767829.1
• Coordinates: 2947441..2947695 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: IQS18_RS14190
• Protein ID: WP_001259255.1
• Coordinates: 2947193..2947444 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS18_RS14165	2942371..2943438	-	1068	WP_000080086.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
IQS18_RS14170	2943438..2944508	-	1071	WP_000108940.1	histidinol-phosphate transaminase	-
IQS18_RS14175	2944505..2945809	-	1305	WP_203024135.1	histidinol dehydrogenase	-
IQS18_RS14180	2945815..2946714	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
IQS18_RS14185	2946860..2946910	-	51	WP_001364200.1	his operon leader peptide	-
IQS18_RS14190	2947193..2947444	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
IQS18_RS14195	2947441..2947695	+	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
IQS18_RS14200	2947778..2948602	+	825	WP_000754737.1	SDR family oxidoreductase	-
IQS18_RS14205	2948648..2949577	+	930	WP_000803360.1	LysR family transcriptional regulator	-
IQS18_RS14210	2949792..2949854	+	63	WP_010723108.1	membrane protein YoeI	-
IQS18_RS14215	2949844..2951202	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
IQS18_RS14220	2951381..2952439	+	1059	WP_203024133.1	YeeE/YedE family protein	-
IQS18_RS14225	2952453..2952680	+	228	WP_000985273.1	sulfurtransferase TusA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T178584 WP_000767829.1 NZ_CP063511:2947441-2947695 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T178584 NZ_CP084168:c1904537-1904190 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGACAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT178584 WP_001259255.1 NZ_CP063511:2947193-2947444 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT178584 NZ_CP084168:c1904190-1903891 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• AlphaFold DB: A0A7U9LNR0