Detailed information of TA system TA178575

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 52874..53415
• Replicon: chromosome
• Accession: NZ_CP063511
• Organism: Escherichia coli strain EH08-18-36

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: IQS18_RS00410
• Protein ID: WP_097331039.1
• Coordinates: 53137..53415 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: S1EWQ0
• Locus tag: IQS18_RS00405
• Protein ID: WP_000729704.1
• Coordinates: 52874..53134 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS18_RS00385	48557..49342	-	786	WP_000207549.1	putative lateral flagellar export/assembly protein LafU	-
IQS18_RS00390	49314..51026	+	1713	Protein_76	flagellar biosynthesis protein FlhA	-
IQS18_RS00395	51242..51739	-	498	WP_203024042.1	REP-associated tyrosine transposase RayT	-
IQS18_RS00400	51915..52688	-	774	WP_203024040.1	C40 family peptidase	-
IQS18_RS00405	52874..53134	+	261	WP_000729704.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
IQS18_RS00410	53137..53415	+	279	WP_097331039.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
IQS18_RS00415	53571..54311	+	741	WP_001225679.1	murein L,D-transpeptidase	-
IQS18_RS00420	54282..55049	-	768	WP_000333379.1	class II glutamine amidotransferase	-
IQS18_RS00425	55255..55833	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-84)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10802.56 Da
• Isoelectric Point: 9.8886

>T178575 WP_097331039.1 NZ_CP063511:53137-53415 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLQ
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T178575 NZ_CP084108:2228740-2229639 [Lysinibacillus capsici]
ATGAATAAGTTTGATCTCGCTTATTTTTCAGAAGATGAGTTCCAGGAACGTTTGCAATTTACCATAGATAGATTAAGTAA
AAAAAAGAAGCCTTTTCAAAAGCCAGTTGCTTATCTTTTAGGGGGACAAGCAGGTGCAGGGAAAACTACATTACATGCTA
TTATAAAGGATAAATTGAACAACGATGTTATAACCATCGATAATGATACCTTTAAACAAATGCATCCTAATTTTGATTTA
CTGGTTGAAAAATACGGTAAAGATTATGTGGCACATGTAACGCCCTTTTCTAACAAAATGACTGAAGCCTTGATTGAACA
TTTTAGTAGCCAGGGTTTCAATTTAACTATTGAAGGCACTTTACGAACAATTGAAACGCCTATGCAGACTGCTACATCTT
TAAAAGAAAAAGGCTATGAAGTCAACCTTTATGCAATGGCAGTATCAAAAGATTTGTCTTATCTAGGTACAATAGAGAGA
TATGAATCAATGTATTTAATCGATCCACAAACAGCAAGATCAACTGATAAGGTTATCCATGACACAATAGTTTCAAATTT
GCCTGATAACCTCGATATGCTTTTTAATAGTGGTTACTTCGCTGAAATTAGTCTTTTCACAAGAGAAGGAACCAAAGTAT
ATTCTAGTGTTGATACGCCTTCAATTAGTCCAAAACAGATTATTTATGAAGCTTTAAATCAAGAAATACCCAAAGATGTG
TTGATTAATAAAATTGATCATATTATCCATTTGACTAAACACAACAATCATCAAGTGCCAGATTTGTTAAATTGGCGAAA
AGAACTGATACGACAATCTATTTCTCCTTTCCAAGACAATGAGAAGAAACAAGACAAAGTATTTAATAAAGTAAATAAAA
ATAAGGTTTTTGAACGTTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9391.77 Da
• Isoelectric Point: 5.0758

>AT178575 WP_000729704.1 NZ_CP063511:52874-53134 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT178575 NZ_CP084108:2228468-2228743 [Lysinibacillus capsici]
ATGGAGAAATCTTTAAATTACGAACAAACTTTTAAAATTGAAGTATTAAACGAATTTTCTAATGGTGTCTATGCACGATT
ACTAAATTTTATCTTGAAAAATGGTGTTGATAAAAACGATAGTAATAACTTTTATGTTAGTCTGTTACAACAGTTCTCAG
AAATGCTTAATGTAGACTTATTTAAAATGTCAAATGTCGAATTAGATAATGTTAAGTCTTATTGGGAGAATATGAACGAT
TACATTAAAGGAATCACAAAGGAAAAATCAGCATGA

Structures

Antitoxin

• PDB: 4ML0
• AlphaFold DB: A0A0E0Y6Z6