Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 46685..47379 Replicon chromosome
Accession NZ_CP063511
Organism Escherichia coli strain EH08-18-36

Toxin (Protein)


Gene name yafO Uniprot ID S1EXB8
Locus tag IQS18_RS00370 Protein ID WP_001263493.1
Coordinates 46685..47083 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag IQS18_RS00375 Protein ID WP_000554757.1
Coordinates 47086..47379 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS18_RS00340 41685..42929 - 1245 WP_000189541.1 esterase FrsA -
- 42345..42425 - 81 NuclAT_8 - -
- 42345..42425 - 81 NuclAT_8 - -
- 42345..42425 - 81 NuclAT_8 - -
- 42345..42425 - 81 NuclAT_8 - -
IQS18_RS00345 43021..43479 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
IQS18_RS00350 43740..45197 + 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
IQS18_RS00355 45254..45775 - 522 Protein_69 peptide chain release factor H -
IQS18_RS00360 45774..45977 - 204 Protein_70 RNA ligase RtcB family protein -
IQS18_RS00365 46223..46675 - 453 WP_001059847.1 GNAT family N-acetyltransferase -
IQS18_RS00370 46685..47083 - 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
IQS18_RS00375 47086..47379 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
IQS18_RS00380 47431..48486 - 1056 WP_001226164.1 DNA polymerase IV -
IQS18_RS00385 48557..49342 - 786 WP_000207549.1 putative lateral flagellar export/assembly protein LafU -
IQS18_RS00390 49314..51026 + 1713 Protein_76 flagellar biosynthesis protein FlhA -
IQS18_RS00395 51242..51739 - 498 WP_203024042.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15472.83 Da        Isoelectric Point: 8.0949

>T178574 WP_001263493.1 NZ_CP063511:c47083-46685 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T178574 NZ_CP084107:c2461507-2461400 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT178574 WP_000554757.1 NZ_CP063511:c47379-47086 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT178574 NZ_CP084107:2461323-2461383 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1EXB8

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References