Detailed information of TA system TA178565

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 105856..106282
• Replicon: plasmid pEH09-18-41_3
• Accession: NZ_CP063506
• Organism: Escherichia coli strain EH09-18-41

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: IQS19_RS24345
• Protein ID: WP_001312861.1
• Coordinates: 106124..106282 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	105856..106080 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS19_RS24310	101555..101761	+	207	WP_000275853.1	hypothetical protein	-
IQS19_RS24315	101787..102326	+	540	WP_032332913.1	single-stranded DNA-binding protein	-
IQS19_RS24320	102383..102616	+	234	WP_000005987.1	DUF905 family protein	-
IQS19_RS24325	102681..104639	+	1959	WP_039000998.1	ParB/RepB/Spo0J family partition protein	-
IQS19_RS24330	104694..105128	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
IQS19_RS24335	105125..105844	+	720	WP_001276232.1	plasmid SOS inhibition protein A	-
IQS19_RS24340	105856..106044	-	189	WP_001299721.1	hypothetical protein	-
-	105856..106080	+	225	NuclAT_0	-	Antitoxin
-	105856..106080	+	225	NuclAT_0	-	Antitoxin
-	105856..106080	+	225	NuclAT_0	-	Antitoxin
-	105856..106080	+	225	NuclAT_0	-	Antitoxin
IQS19_RS24345	106124..106282	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
IQS19_RS24350	107204..107491	+	288	WP_000107535.1	hypothetical protein	-
IQS19_RS24355	107613..108434	+	822	WP_072795862.1	DUF945 domain-containing protein	-
IQS19_RS24360	108731..109333	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
IQS19_RS24365	109654..110037	+	384	WP_001151524.1	relaxosome protein TraM	-
IQS19_RS24370	110224..110913	+	690	WP_039002187.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IId / mcr-3.1 / blaTEM-1B / sul3 / tet(A) / sul2 / floR / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / tet(M)	-	1..131695	131695

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T178565 WP_001312861.1 NZ_CP063506:106124-106282 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T178565 NZ_CP084107:349973-350080 [Staphylococcus aureus]
GTGGTATCTATTGTTGATGCGCTAAATTTGATGTTTAGTTTCGGTATGTTTATCGTTACTTTACTTGGTTTGGTCATCGC
AATTGTTAAATTAAACCATAAAAAATAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT178565 NZ_CP063506:105856-106080 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin