Detailed information of TA system TA178560

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 9632..9886
• Replicon: plasmid pEH09-18-41_3
• Accession: NZ_CP063506
• Organism: Escherichia coli strain EH09-18-41

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: IQS19_RS23760
• Protein ID: WP_001351576.1
• Coordinates: 9680..9886 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	9632..9693 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS19_RS23745	7585..8328	+	744	WP_039002216.1	conjugal transfer pilus acetylase TraX	-
IQS19_RS23750	8383..8943	+	561	WP_000139310.1	fertility inhibition protein FinO	-
IQS19_RS23755	9078..9290	+	213	WP_001541896.1	ANR family transcriptional regulator	-
-	9632..9693	-	62	NuclAT_1	-	Antitoxin
-	9632..9693	-	62	NuclAT_1	-	Antitoxin
-	9632..9693	-	62	NuclAT_1	-	Antitoxin
-	9632..9693	-	62	NuclAT_1	-	Antitoxin
IQS19_RS23760	9680..9886	+	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
IQS19_RS23765	10154..10411	+	258	WP_001541895.1	replication regulatory protein RepA	-
IQS19_RS23770	10643..10717	+	75	WP_061357229.1	RepA leader peptide Tap	-
IQS19_RS23775	10710..11567	+	858	WP_039002220.1	incFII family plasmid replication initiator RepA	-
IQS19_RS23780	12533..12784	+	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IQS19_RS23785	12781..13068	+	288	WP_000222766.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IQS19_RS23790	13143..13331	-	189	Protein_11	IS3 family transposase	-
IQS19_RS23795	13365..13865	-	501	Protein_12	Tn3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IId / mcr-3.1 / blaTEM-1B / sul3 / tet(A) / sul2 / floR / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / tet(M)	-	1..131695	131695

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T178560 WP_001351576.1 NZ_CP063506:9680-9886 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T178560 NZ_CP084103:c4739085-4738168 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCAATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGTTGAAGTGCTGAGTCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT178560 NZ_CP063506:c9693-9632 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin