Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3050959..3051461 Replicon chromosome
Accession NZ_CP063503
Organism Escherichia coli strain EH09-18-41

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag IQS19_RS14830 Protein ID WP_000767829.1
Coordinates 3050959..3051213 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag IQS19_RS14835 Protein ID WP_001259255.1
Coordinates 3051210..3051461 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS19_RS14800 3045974..3046201 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
IQS19_RS14805 3046215..3047273 - 1059 WP_000492325.1 transport protein YeeE -
IQS19_RS14810 3047452..3048810 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
IQS19_RS14815 3048800..3048862 - 63 WP_010723108.1 membrane protein YoeI -
IQS19_RS14820 3049077..3050006 - 930 WP_000803351.1 LysR family transcriptional regulator -
IQS19_RS14825 3050052..3050876 - 825 WP_000754737.1 SDR family oxidoreductase -
IQS19_RS14830 3050959..3051213 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
IQS19_RS14835 3051210..3051461 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
IQS19_RS14840 3051744..3051794 + 51 WP_001364200.1 his operon leader peptide -
IQS19_RS14845 3051940..3052839 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
IQS19_RS14850 3052845..3054149 + 1305 WP_001295420.1 histidinol dehydrogenase -
IQS19_RS14855 3054146..3055216 + 1071 WP_203152312.1 histidinol-phosphate transaminase -
IQS19_RS14860 3055216..3056283 + 1068 WP_000080067.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T178552 WP_000767829.1 NZ_CP063503:c3051213-3050959 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T178552 NZ_CP084103:323505-323873 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT178552 WP_001259255.1 NZ_CP063503:c3051461-3051210 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT178552 NZ_CP084103:323287-323508 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References