Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1104949..1105643 Replicon chromosome
Accession NZ_CP063503
Organism Escherichia coli strain EH09-18-41

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag IQS19_RS05185 Protein ID WP_001263489.1
Coordinates 1105245..1105643 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag IQS19_RS05180 Protein ID WP_000554758.1
Coordinates 1104949..1105242 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS19_RS05160 1100682..1101179 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
IQS19_RS05165 1101302..1103014 - 1713 Protein_989 flagellar biosynthesis protein FlhA -
IQS19_RS05170 1102986..1103771 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
IQS19_RS05175 1103842..1104897 + 1056 WP_001226164.1 DNA polymerase IV -
IQS19_RS05180 1104949..1105242 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
IQS19_RS05185 1105245..1105643 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
IQS19_RS05190 1105653..1106105 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
IQS19_RS05195 1106423..1106629 + 207 Protein_995 RtcB family protein -
IQS19_RS05200 1106625..1107146 + 522 Protein_996 peptide chain release factor H -
IQS19_RS05205 1107203..1108660 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
IQS19_RS05210 1108921..1109379 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 1109975..1110055 + 81 NuclAT_11 - -
- 1109975..1110055 + 81 NuclAT_11 - -
- 1109975..1110055 + 81 NuclAT_11 - -
- 1109975..1110055 + 81 NuclAT_11 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T178539 WP_001263489.1 NZ_CP063503:1105245-1105643 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T178539 NZ_CP084096:c3594248-3593994 [Escherichia coli O78]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT178539 WP_000554758.1 NZ_CP063503:1104949-1105242 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT178539 NZ_CP084096:c3594496-3594245 [Escherichia coli O78]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References