Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1099006..1099547 Replicon chromosome
Accession NZ_CP063503
Organism Escherichia coli strain EH09-18-41

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag IQS19_RS05145 Protein ID WP_000615983.1
Coordinates 1099006..1099284 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag IQS19_RS05150 Protein ID WP_000729703.1
Coordinates 1099287..1099547 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS19_RS05130 1096588..1097166 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
IQS19_RS05135 1097372..1098139 + 768 WP_000333380.1 class II glutamine amidotransferase -
IQS19_RS05140 1098110..1098850 - 741 WP_001225679.1 murein L,D-transpeptidase -
IQS19_RS05145 1099006..1099284 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
IQS19_RS05150 1099287..1099547 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
IQS19_RS05155 1099757..1100506 + 750 WP_000056849.1 C40 family peptidase -
IQS19_RS05160 1100682..1101179 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
IQS19_RS05165 1101302..1103014 - 1713 Protein_989 flagellar biosynthesis protein FlhA -
IQS19_RS05170 1102986..1103771 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T178538 WP_000615983.1 NZ_CP063503:c1099284-1099006 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T178538 NZ_CP084096:3580459-3580833 [Escherichia coli O78]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCATCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT178538 WP_000729703.1 NZ_CP063503:c1099547-1099287 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT178538 NZ_CP084096:3580002-3580370 [Escherichia coli O78]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCACCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References