Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4097810..4098609 Replicon chromosome
Accession NZ_CP063499
Organism Escherichia coli strain EH10-18-47

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag IQS20_RS19690 Protein ID WP_000347273.1
Coordinates 4097810..4098274 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag IQS20_RS19695 Protein ID WP_001307405.1
Coordinates 4098274..4098609 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS20_RS19660 4092811..4093245 - 435 WP_000948814.1 PTS sugar transporter subunit IIA -
IQS20_RS19665 4093263..4094141 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
IQS20_RS19670 4094131..4094910 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
IQS20_RS19675 4094921..4095394 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
IQS20_RS19680 4095417..4096697 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
IQS20_RS19685 4096946..4097755 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
IQS20_RS19690 4097810..4098274 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
IQS20_RS19695 4098274..4098609 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
IQS20_RS19700 4098758..4100329 - 1572 WP_001273753.1 galactarate dehydratase -
IQS20_RS19705 4100704..4102038 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
IQS20_RS19710 4102054..4102824 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T178529 WP_000347273.1 NZ_CP063499:c4098274-4097810 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T178529 NZ_CP084096:c1525772-1525494 [Escherichia coli O78]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATATTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT178529 WP_001307405.1 NZ_CP063499:c4098609-4098274 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT178529 NZ_CP084096:c1526035-1525775 [Escherichia coli O78]
ATGGCTGCAAATGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References