Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2454331..2454872 Replicon chromosome
Accession NZ_CP063499
Organism Escherichia coli strain EH10-18-47

Toxin (Protein)


Gene name yafQ Uniprot ID A0A0K4UG80
Locus tag IQS20_RS11920 Protein ID WP_033802988.1
Coordinates 2454594..2454872 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID A0A0K5TST2
Locus tag IQS20_RS11915 Protein ID WP_033802987.1
Coordinates 2454331..2454591 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS20_RS11895 2449804..2450589 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
IQS20_RS11900 2450561..2452273 + 1713 Protein_2331 flagellar biosynthesis protein FlhA -
IQS20_RS11905 2452699..2453196 - 498 WP_033802985.1 REP-associated tyrosine transposase RayT -
IQS20_RS11910 2453372..2454121 - 750 WP_033802986.1 C40 family peptidase -
IQS20_RS11915 2454331..2454591 + 261 WP_033802987.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
IQS20_RS11920 2454594..2454872 + 279 WP_033802988.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
IQS20_RS11925 2455028..2455768 + 741 WP_001225679.1 murein L,D-transpeptidase -
IQS20_RS11930 2455739..2456506 - 768 WP_000333380.1 class II glutamine amidotransferase -
IQS20_RS11935 2456712..2457290 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA 2402361..2479658 77297


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10828.65 Da        Isoelectric Point: 10.0702

>T178526 WP_033802988.1 NZ_CP063499:2454594-2454872 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAVLFG

Download         Length: 279 bp

>T178526 NZ_CP084096:1059590-1059940 [Escherichia coli O78]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9390.79 Da        Isoelectric Point: 5.8129

>AT178526 WP_033802987.1 NZ_CP063499:2454331-2454591 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKNADDL
FDKLGI

Download         Length: 261 bp

>AT178526 NZ_CP084096:1059345-1059596 [Escherichia coli O78]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K4UG80

Antitoxin

Source ID Structure
AlphaFold DB A0A0K5TST2

References