Detailed information of TA system TA178525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2447932..2448626
• Replicon: chromosome
• Accession: NZ_CP063499
• Organism: Escherichia coli strain EH10-18-47

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: IQS20_RS11880
• Protein ID: WP_001263493.1
• Coordinates: 2447932..2448330 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: IQS20_RS11885
• Protein ID: WP_000554757.1
• Coordinates: 2448333..2448626 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS20_RS11850	2442932..2444176	-	1245	WP_000189541.1	esterase FrsA	-
-	2443592..2443672	-	81	NuclAT_9	-	-
-	2443592..2443672	-	81	NuclAT_9	-	-
-	2443592..2443672	-	81	NuclAT_9	-	-
-	2443592..2443672	-	81	NuclAT_9	-	-
IQS20_RS11855	2444268..2444726	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
IQS20_RS11860	2444987..2446444	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
IQS20_RS11865	2446501..2447022	-	522	Protein_2324	peptide chain release factor H	-
IQS20_RS11870	2447021..2447224	-	204	Protein_2325	RNA ligase RtcB family protein	-
IQS20_RS11875	2447470..2447922	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
IQS20_RS11880	2447932..2448330	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
IQS20_RS11885	2448333..2448626	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
IQS20_RS11890	2448678..2449733	-	1056	WP_001226164.1	DNA polymerase IV	-
IQS20_RS11895	2449804..2450589	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
IQS20_RS11900	2450561..2452273	+	1713	Protein_2331	flagellar biosynthesis protein FlhA	-
IQS20_RS11905	2452699..2453196	-	498	WP_033802985.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA	2402361..2479658	77297

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T178525 WP_001263493.1 NZ_CP063499:c2448330-2447932 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T178525 NZ_CP084096:c881305-880928 [Escherichia coli O78]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT178525 WP_000554757.1 NZ_CP063499:c2448626-2448333 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT178525 NZ_CP084096:c881729-881352 [Escherichia coli O78]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGAGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAATCACCGTATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6