Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 609419..609921 Replicon chromosome
Accession NZ_CP063499
Organism Escherichia coli strain EH10-18-47

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag IQS20_RS02755 Protein ID WP_000767829.1
Coordinates 609667..609921 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag IQS20_RS02750 Protein ID WP_001259255.1
Coordinates 609419..609670 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS20_RS02725 604596..605663 - 1068 WP_000080097.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
IQS20_RS02730 605663..606733 - 1071 WP_000108937.1 histidinol-phosphate transaminase -
IQS20_RS02735 606730..608034 - 1305 WP_001372394.1 histidinol dehydrogenase -
IQS20_RS02740 608040..608939 - 900 WP_000131776.1 ATP phosphoribosyltransferase -
IQS20_RS02745 609085..609135 - 51 WP_001364200.1 his operon leader peptide -
IQS20_RS02750 609419..609670 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
IQS20_RS02755 609667..609921 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
IQS20_RS02760 610004..610828 + 825 WP_000754737.1 SDR family oxidoreductase -
IQS20_RS02765 610874..611803 + 930 WP_000803351.1 LysR family transcriptional regulator -
IQS20_RS02770 612018..612080 + 63 WP_010723108.1 membrane protein YoeI -
IQS20_RS02775 612070..613428 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
IQS20_RS02780 613607..614665 + 1059 WP_000492312.1 transport protein YeeE -
IQS20_RS02785 614679..614906 + 228 WP_000985278.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T178513 WP_000767829.1 NZ_CP063499:609667-609921 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T178513 NZ_CP084055:3507563-3507850 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGACATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT178513 WP_001259255.1 NZ_CP063499:609419-609670 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT178513 NZ_CP084055:3507837-3508139 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References