Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 5251203..5252002 Replicon chromosome
Accession NZ_CP063492
Organism Escherichia coli strain EF5-18-41

Toxin (Protein)


Gene name yhaV Uniprot ID B7NDB6
Locus tag IQS14_RS25500 Protein ID WP_000347269.1
Coordinates 5251538..5252002 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID V0YUB5
Locus tag IQS14_RS25495 Protein ID WP_001309780.1
Coordinates 5251203..5251538 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS14_RS25480 5246988..5247758 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
IQS14_RS25485 5247774..5249108 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
IQS14_RS25490 5249483..5251054 + 1572 WP_001273738.1 galactarate dehydratase -
IQS14_RS25495 5251203..5251538 + 336 WP_001309780.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
IQS14_RS25500 5251538..5252002 + 465 WP_000347269.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
IQS14_RS25505 5252057..5252866 - 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
IQS14_RS25510 5253115..5254395 + 1281 WP_000681943.1 tagatose-bisphosphate aldolase subunit KbaZ -
IQS14_RS25515 5254418..5254891 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
IQS14_RS25520 5254902..5255681 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
IQS14_RS25525 5255671..5256549 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
IQS14_RS25530 5256567..5257001 + 435 WP_000948818.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 5241961..5252002 10041


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T178503 WP_000347269.1 NZ_CP063492:5251538-5252002 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTREAEETH

Download         Length: 465 bp

>T178503 NZ_CP084055:517325-517501 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12345.94 Da        Isoelectric Point: 4.8616

>AT178503 WP_001309780.1 NZ_CP063492:5251203-5251538 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNT
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT178503 NZ_CP084055:517547-517963 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829IY86

Antitoxin

Source ID Structure
AlphaFold DB V0YUB5

References