Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3169963..3170525 Replicon chromosome
Accession NZ_CP063492
Organism Escherichia coli strain EF5-18-41

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag IQS14_RS15365 Protein ID WP_000605676.1
Coordinates 3170247..3170525 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag IQS14_RS15360 Protein ID WP_000781370.1
Coordinates 3169963..3170247 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS14_RS15345 3165145..3168192 + 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -
IQS14_RS15350 3168205..3169089 + 885 WP_001240565.1 formate dehydrogenase N subunit beta -
IQS14_RS15355 3169082..3169735 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
IQS14_RS15360 3169963..3170247 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
IQS14_RS15365 3170247..3170525 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IQS14_RS15370 3170711..3171721 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
IQS14_RS15375 3171855..3173552 - 1698 WP_000433464.1 malate dehydrogenase -
IQS14_RS15380 3173709..3173846 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
IQS14_RS15385 3173948..3174163 - 216 WP_000495766.1 biofilm-dependent modulation protein -
IQS14_RS15390 3174508..3174939 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T178487 WP_000605676.1 NZ_CP063492:c3170525-3170247 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T178487 NZ_CP084032:c4459733-4459248 [Pectobacterium colocasium]
ATGGTTGATAAGCGAGTAATAAATGGGTGGCAGCTATTTACGCATCCTTGTTTTGATGTGCAGTTTGAGTCTCTTATTGT
TGAAGTTGAACAATTGAGAAAAAAACACCCAGAATCTTATCAGAAAAAGCAGGCAACTAAGCTATTGGCTGCAATAGTCA
AAGTCATCCGTACTGAGATTTGTGGCGATCCGTCCCAGACGAAGTTTAGACAAGGCGATACGTTAGGCGAAGGCAACAAA
CATTGGTTCAGGGCAAAATTTCTGGGTCAGTATCGGTTATTTTTTCGCTATAGCGAGAAACATAAAACTATCATCCTTGC
CTGGGTCAATGATTCAGACACTAAACGAGCATATGGCGATCAGCATGATGCTTATAAAGTTTTTAGCGGCATGCTAGCAA
GCGGGAATCCTCCGGGAGATTGGAAGCAATTGCTTGCTGAATCCTATAAGGAGACCATGCTTACGCAAATGCTCGTGCGG
CCATAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT178487 WP_000781370.1 NZ_CP063492:c3170247-3169963 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT178487 NZ_CP084032:c4460083-4459739 [Pectobacterium colocasium]
ATGTTTAATTTAAAATCAGCGGATGTATTGTTGCGCGCCGATAGCCGCTTAACGGCGCGTAGTCAGACAACGATTCCATC
GTCTGTGCGTGATGCCATGAACCTGCAACCTGGGGAGTACATTCATTATAGCGTGCTACCGGGTGGGCAGGTGCTGATCA
GTCGGCGTGAAGAGGCTGTAGATGACGATCCCGTTATTCATCGTTTTCTGCATTTTTTGGCTGAAGATATGCAACATCAT
CCAGAAAATATTAAGCCATTTGATCAATCGCTTATGTCACGTGCCAGTGTGTTAACCGCAGGGATGGATATTGATCTGGA
TGCACCGCTTACTGACGACGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References