Detailed information of TA system TA178478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1755189..1755839
• Replicon: chromosome
• Accession: NZ_CP063492
• Organism: Escherichia coli strain EF5-18-41

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: IQS14_RS08300
• Protein ID: WP_000263532.1
• Coordinates: 1755189..1755530 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: U9YXQ7
• Locus tag: IQS14_RS08305
• Protein ID: WP_000212552.1
• Coordinates: 1755540..1755839 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS14_RS08275	1750224..1751324	+	1101	WP_001181876.1	flagellar basal body P-ring protein FlgI	-
IQS14_RS08280	1751324..1751623	+	300	WP_000867281.1	rod-binding protein	-
IQS14_RS08285	1751799..1753175	+	1377	WP_000367245.1	flagellar hook-associated protein FlgK	-
IQS14_RS08290	1753190..1754119	+	930	WP_001266799.1	flagellar hook-associated protein FlgL	-
IQS14_RS08295	1754136..1755113	+	978	WP_001195938.1	hypothetical protein	-
IQS14_RS08300	1755189..1755530	+	342	WP_000263532.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IQS14_RS08305	1755540..1755839	+	300	WP_000212552.1	helix-turn-helix transcriptional regulator	Antitoxin
IQS14_RS08310	1755897..1756739	-	843	WP_000022366.1	winged helix-turn-helix domain-containing protein	-
IQS14_RS08315	1757225..1758139	+	915	WP_000949083.1	lateral flagellin LafA	-
IQS14_RS08320	1758344..1759660	+	1317	WP_000609678.1	flagellar filament capping protein FliD	-
IQS14_RS08325	1759683..1760075	+	393	WP_000285322.1	flagellar export chaperone FliS	-
IQS14_RS08330	1760080..1760391	+	312	WP_000415004.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13031.86 Da
• Isoelectric Point: 6.4677

>T178478 WP_000263532.1 NZ_CP063492:1755189-1755530 [Escherichia coli]
MWDVETTETFDNWFDAQTVALKEDLLAAMLILAEYGPQLGRPFADTVNDSQFSNMKELRVQHQGNPVRAFFAFDPARRGI
VLCAGDKTGLNEKKFYRDMIKLADSEYRKHLKK

Nucleotide

• Length: 342 bp

>T178478 NZ_CP084032:c511297-511046 [Pectobacterium colocasium]
ATGCGTACAATTAGCTACAGTGAAGCCCGACAAAATCTATCGGCGACCATGATGAAAACGGTCGAAGACCGTGCTCCTAT
TCTTATCACTCGACAGAATGGTGAAGCCTGCGTGCTGATGTCTTTGGAAGAGTATAACTCTCTAGAAGAAACCGCGTATT
TACTGCGTTCACCAGCCAATGCCAAAAGACTGATGAACTCAATCGAGAGCCTTAAGGCTGGCCATGGCGAAGAAAGAGAT
ATCATTGAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11004.86 Da
• Isoelectric Point: 9.7665

>AT178478 WP_000212552.1 NZ_CP063492:1755540-1755839 [Escherichia coli]
MATLKELMAKQSPESQVRIAAKVEEMRQTIALHQLREELNISQTEMAAAMGIKQPTLAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT178478 NZ_CP084032:c511049-510795 [Pectobacterium colocasium]
GTGAAACTAATCTGGTCAGAAGAAGCATGGGAAGACTACCTGTACTGGCAGGACATCGATAAGCGGATGGTCAAAAAGAT
CAATGAATTAATAAAAGAAACGCGCAGAACACCTTTTGAGGGAAAAGGAAAACCAGAGCCACTTAAACATAACCTTGCTG
GTTTCTGGTCGCGCCGTATCACCGAAGAACATCGTTTGGTTTATGCCATCACCGATGACGCCATGATGATCGCTGCCTGC
CGCTACCACTATTAA

Structures

Antitoxin

• AlphaFold DB: U9YXQ7