Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2499754..2500481 Replicon chromosome
Accession NZ_CP063487
Organism Escherichia coli strain EF7-18-51

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag IQS15_RS12030 Protein ID WP_000550189.1
Coordinates 2499754..2500068 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag IQS15_RS12035 Protein ID WP_097402472.1
Coordinates 2500065..2500481 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS15_RS12010 2495912..2496898 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
IQS15_RS12015 2496977..2497669 - 693 Protein_2327 vancomycin high temperature exclusion protein -
IQS15_RS12020 2497746..2498249 - 504 WP_001333820.1 M48 family metallopeptidase -
IQS15_RS12025 2498334..2499470 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
IQS15_RS12030 2499754..2500068 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
IQS15_RS12035 2500065..2500481 + 417 WP_097402472.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
IQS15_RS12040 2500526..2502544 - 2019 WP_072712650.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
IQS15_RS12045 2502970..2505321 - 2352 WP_072712653.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T178447 WP_000550189.1 NZ_CP063487:2499754-2500068 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T178447 NZ_CP084026:1656166-1656417 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15025.44 Da        Isoelectric Point: 4.4547

>AT178447 WP_097402472.1 NZ_CP063487:2500065-2500481 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHTKKLATRFGISPALFID

Download         Length: 417 bp

>AT178447 NZ_CP084026:1656414-1656668 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References