Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4374704..4375245 Replicon chromosome
Accession NZ_CP063483
Organism Escherichia coli strain EM03-18-08

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag IQS21_RS21015 Protein ID WP_000615983.1
Coordinates 4374967..4375245 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag IQS21_RS21010 Protein ID WP_000729703.1
Coordinates 4374704..4374964 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS21_RS20990 4370379..4371164 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
IQS21_RS20995 4371136..4372848 + 1713 Protein_4087 flagellar biosynthesis protein FlhA -
IQS21_RS21000 4373072..4373569 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
IQS21_RS21005 4373745..4374494 - 750 WP_000056849.1 C40 family peptidase -
IQS21_RS21010 4374704..4374964 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
IQS21_RS21015 4374967..4375245 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
IQS21_RS21020 4375401..4376141 + 741 WP_001225679.1 murein L,D-transpeptidase -
IQS21_RS21025 4376112..4376879 - 768 WP_000333380.1 class II glutamine amidotransferase -
IQS21_RS21030 4377085..4377663 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T178433 WP_000615983.1 NZ_CP063483:4374967-4375245 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T178433 NZ_CP084023:c4013541-4013128 [Pectobacterium aroidearum]
GTGGCCCAGTGGCAATGTGATCTTCGCGTTTCCTGGCGTATGCAACTGCTTTCATTGATTGTGCATGGCCTATTAGTATT
GCTCATTCTTCTGGCTCCGTGGCCGGACGGCTATGCATGGCTGTGGCTATGCCTGGTCACGATGGTGATGTTCGGTTTTA
TCCGTAGCCAAAGGAATATCAAATCCCGGCAGGGGGAAATCGTTTTGCTCAGCGAAACGACCCTGAACTGGCGGCAGCAG
GAATGGCAGATCGTTAAACGACCGTGGTTGCTGAAAAATGGCGTATTGCTGTCATTGCAGGCAGTGAACGGTAAAGACAG
ACAGCAGCTGTGGCTGGCATCGGACAGCATGGGCGATGACGAATGGCGTCATCTGCGTCAGCTTCTTTTGCAGCAGAAAA
ACTGGGCGAGATAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT178433 WP_000729703.1 NZ_CP063483:4374704-4374964 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT178433 NZ_CP084023:c4013788-4013522 [Pectobacterium aroidearum]
ATGGAAATCGATAATAAATCACGCATTCATTGGGCATGCCGCCGGGGTATGCGTGAGTTGGATATCGCGATCATGCCGTT
TTTTGAGCATGACTACGACACACTCAGTGACAGCGATAAGCGCACGTTTGTGCGCCTGTTACAAAGCGACGATCCCGATT
TATTCAACTGGCTGATGAACCACGGCGAGCCGAAAGACGGAGAGCTGAAACGCATGATTTCTCTTATTCAAACGCGAAAT
AAAGATCGTGGCCCAGTGGCAATGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References